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Functional and phenotypic characterization of 2 hl 60 clones resistant to di methyl sulfoxide


, : Functional and phenotypic characterization of 2 hl 60 clones resistant to di methyl sulfoxide. Experimental Cell Research 147(1): 111-118

Two HL60 clones (C12 and C13) totally insensitive to differentiation induction by dimethylsulfoxide (Me2SO) are described. They have been growing continuously in the presence of the inducer for more than 6 mo. Morphological and cytochemical features of the 2 populations are quite similar to those of the original HL60 cell line; a different karyotype with marked hyperploidy (modal chromosome number of 86 for C12 and 82 for C13) was detected. An antigenic pattern analogous to that of the native HL60 cell line was found in C12 and C13 populations using 3 monoclonal antibodies differently reactive to myeloid cells. Both clones can be induced to differentiate by retinoic acid (RA) and 12-O-tetradecanoylphorbol 13-acetate (TPA). The pattern of differentiation was assessed by morphological, cytochemical, phenotypical and functional markers. Differentiation of C12 cells by RA and TPA was similar to that observed with native HL60 cells; C13 cells showed lower degrees of sensitivity to RA and TPA. Data suggest the existence of different mechanisms for induction of differentiation by Me2SO, RA and TPA. They are also in accordance with previous observations of different degrees of inducibility to differentiation among leukemic cell populations in culture.

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