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Further studies on the production of dengue virus induced macrophage cyto toxin

, : Further studies on the production of dengue virus induced macrophage cyto toxin. Indian Journal of Experimental Biology 21(5): 275-279

Dengue virus-induced cytotoxic factor (CF) triggers normal mouse splenic and peritoneal macrophages to produce a cytotoxin (CF2). A very small quantity of CF is required to induce macrophages and at least 5 .times. 105 macrophages must be used to produce detectable amounts of CF2. The shortest period required for the induction is 45 min and the CF2 remains in the macrophages up to 24 h. Induction of CF2 is independent of the strain of animals or their genetic constitution. CF2 production can also be induced by spleen cells capable of secreting CF. The macrophages become refractory to reinduction up to 3 days; they regain the capacity and can be reinduced by CF on the 4th day of cultures to produce fresh CF2.


PMID: 6667980

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