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Genetic changes in yeast saccharomyces cerevisiae cells co transformed with mitochondrial dna and plasmid yep 13 are not elicited by recombinant molecules made by covalent insertion of mitochondrial dna into yep 13


, : Genetic changes in yeast saccharomyces cerevisiae cells co transformed with mitochondrial dna and plasmid yep 13 are not elicited by recombinant molecules made by covalent insertion of mitochondrial dna into yep 13. Biochemical & Biophysical Research Communications 109(2): 455-462

S. cerevisiae strain DC5-E2 that lacks mtDNA (leu2 rho.degree.) can be cotransformed with a mixture of mtDNA and the plasmid YEp13 (LEU2/2.mu./pBR322) to produce Leu+ transformants which, on being mated to mit- tester strains, generate respiratory competent diploids (such events are denoted marker rescue). Strain DC5-E2 was transformed with recombinant molecules consisting of a mtDNA segment including the oli2 gene inserted into YEp13. Leu+ transformants made with the recombinant plasmids were unable to rescue mit- testers carrying mutations in the oli2 region, in contrast to Leu+ cotransformants made with mixtures of YEp13 and oli2 mtDNA. Marker rescue events occur as a result of interactions between the mtDNA of the mit- tester and the mtDNA sequences introduced by transformation. Such interactions cannot occur when the latter mtDNA is forced to replicate in covalent association with YEp13, probably in the nucleus.

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