+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Genetic changes in yeast saccharomyces cerevisiae cells co transformed with mitochondrial dna and plasmid yep 13 are not elicited by recombinant molecules made by covalent insertion of mitochondrial dna into yep 13

, : Genetic changes in yeast saccharomyces cerevisiae cells co transformed with mitochondrial dna and plasmid yep 13 are not elicited by recombinant molecules made by covalent insertion of mitochondrial dna into yep 13. Biochemical & Biophysical Research Communications 109(2): 455-462

S. cerevisiae strain DC5-E2 that lacks mtDNA (leu2 can be cotransformed with a mixture of mtDNA and the plasmid YEp13 (LEU2/ to produce Leu+ transformants which, on being mated to mit- tester strains, generate respiratory competent diploids (such events are denoted marker rescue). Strain DC5-E2 was transformed with recombinant molecules consisting of a mtDNA segment including the oli2 gene inserted into YEp13. Leu+ transformants made with the recombinant plasmids were unable to rescue mit- testers carrying mutations in the oli2 region, in contrast to Leu+ cotransformants made with mixtures of YEp13 and oli2 mtDNA. Marker rescue events occur as a result of interactions between the mtDNA of the mit- tester and the mtDNA sequences introduced by transformation. Such interactions cannot occur when the latter mtDNA is forced to replicate in covalent association with YEp13, probably in the nucleus.


Other references

Can, S.Meltem.; Aydin, Y.; Turkmenoglu, O.; Aydin, F.; Ziyal, I., 2003: Giant cell glioblastoma manifesting as traumatic intracerebral hemorrhage--case report. A 33-year-old male presented with intracerebral hemorrhage in the left temporoparietal region after a traffic accident. Ten months later, the traumatic hemorrhage was found to originate in an underlying giant cell glioblastoma. Our case indicates...

Wasmund, E., 1928: Insekten-Massenschwarme am Bodensee und in Nurnberg. Zeitschrift fuer Wissenschaftliche Insektenbiologie Berlin, 23: 234-243

Vogel, M.; Staller, W.; Bühlmeyer, K.; Sebening, F., 1992: Influence of age at time of surgery on pre-operative left ventricular mass and postoperative outcome of Fontan operation in children with tricuspid atresia and native pulmonary stenosis. Purpose of this study was to examine the influence of early ( lt two and half years) versus later ( gt four years) age at time of Fontan type palliation in tricuspid atresia with native pulmonary stenosis on outcome with special reference to left...

Reginato, A.J., 1997: Musculoskeletal manifestations of osteomalacia. Revue du Rhumatisme 64(6 Suppl): 107s-113s

Brumpt, E.; Langeron, M., 1934: Considerations on the South American ' piedra ' in connexion with a case from Venezuela. Description of a new species, Piedraia venezuelensis n. sp. In this paper the authors draw a clear distinction between two types of ' piedra ', the one of which, due to species of Trichosporum, chiefly occurs in the Old World and is characterized by light-coloured nodules on the affected hairs, c...

Rabinak, C.A.; Angstadt, M.; Welsh, R.C.; Kenndy, A.E.; Lyubkin, M.; Martis, B.; Phan, K.Luan., 2011: Altered amygdala resting-state functional connectivity in post-traumatic stress disorder. Post-traumatic stress disorder (PTSD) is often characterized by aberrant amygdala activation and functional abnormalities in corticolimbic circuitry, as elucidated by functional neuroimaging. These "activation" studies have primarily rel...

Shimada, K.; Asahina, E., 1972: Types of cell freezing and postthawing survival of individual HeLa cells. Cryobiology 9(1): 51-56

Hung, O.L.; Shih, R.D., 2010: Antiepileptic drugs: the old and the new. During the past decade several new antiepileptic drugs (AEDs) have become available, including new formulations of some of the older medications. Understanding the pharmacokinetics of the new AEDs is important because they are primarily used for a...

Basu, M.K.; Dutta, A.N., 1965: Report on prevalence of periodontal disease in adult population at Calcutta--Russel's technique. Journal of the All India Dental Association 37(7): 230-3 Passim

Saha, B.; Evers, T.H.; Prins, M.W.J., 2015: How antibody surface coverage on nanoparticles determines the activity and kinetics of antigen capturing for biosensing. The antigen-capturing activity of antibody-coated nanoparticles is very important for affinity-based bioanalytical tools. In this paper, a comprehensive study is reported of the antigen-capturing activity of antibodies that are nondirectionally im...