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Growth of dense populations of acanthamoeba castellanii in small volumes of nutrient and the influence of 4' 6 di amidino 2 phenyl indole a dna ligand on such populations


, : Growth of dense populations of acanthamoeba castellanii in small volumes of nutrient and the influence of 4' 6 di amidino 2 phenyl indole a dna ligand on such populations. Acta Protozoologica 21(2): 157-162

A freshly inoculated population of A. castellanii started with a high number of cells growing in small volumes of nutrient can undergo a marked fall in cell number before it begins to proliferate in the new environment. Also, the general outlines of DAPI [4'-6-diamidino-2-phenylindole] influence on cell proliferation inhibiting the growth of the populations were confirmed. DAPI reduces the accessibility of DNA for nucleic acid polymerases. In this way DAPI hampers transcription on DNA. DAPI inhibits growth in cell populations such as paramecium. DAPI forms also intensely fluorizing complexes with the DNA double helix, in nuclei and in other cell structures which possess DNA. These properties of DAPI should bear on the growth pattern of developing populations of cells such as A. castellanii.

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