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Heterogeneity of the free hemo globins of propsilocerus danubialis diptera chironomidae


, : Heterogeneity of the free hemo globins of propsilocerus danubialis diptera chironomidae. Revue Roumaine de Biologie Serie de Biologie Animale 25(2): 127-132

In contrast with other species of chironomids (9-12 components) a lower heterogeneity (4 components) of free Hb from the hemolymph of P. danubialis larvae was revealed. A monomeric and polymorphic Hb (Hb 2, a and b components) and a dimeric and polymorphic one (HB 1, aa and bb components) with an estimated MW of 16,000 and 32,000, respectively, were isolated by gel filtration (Sephadex G-75) and polyacrylamide electrophoresis before and after treatment with mercaptoethanol and dodecylsulfate. The marked tendency to form a dimeric structure can explain the incapacity of these larvae to take up O2 when its partial pressure is lower than 10 mm Hg.

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