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High performance liquid chromatographic separation of natural adenosyl sulfur compounds

, : High performance liquid chromatographic separation of natural adenosyl sulfur compounds. Journal of Chromatography 189(3): 399-405

A rapid, sensitive and specific high-performance liquid chromatographic method for the simultaneous separation of natural adenosyl-sulfur compounds was developed. The compounds were separated by using the strong cation-exchange resin Partisil 10 SCX with isocratic elution. The adenosyl-compounds were monitored by a UV detector operating at 254 nm. Sensitivity was greater than 50 pmol for all compounds tested and standard curves were linear for concentrations of up to 50 nmol. The method can be applied to biological samples for the estimation of [Saccharomyces cerevisiae] S-adenosyl-L-methionine, S-adenosyl-L-homocysteine and S-adenosyl-(5')-3-methyl-thiopropylamine and for measurement of enzyme activities involving the above-mentioned compounds.


PMID: 7380939

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