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Hysteresis of mushroom agaricus bisporus tyrosinase ec 1.14.18.1 lag period of cresolase activity


, : Hysteresis of mushroom agaricus bisporus tyrosinase ec 1.14.18.1 lag period of cresolase activity. Phytochemistry (Oxford) 20(6): 1215-1218

Mushroom [A. bispora] tyrosinase presents a lag period in the expression of its cresolase activity depending on enzyme and substrate concentration in the reaction medium. At a fixed enzyme concentration in the reaction medium, the lag period depends on the concentration of previously stored enzyme. Experimental data are in agreement with the existence of a hysteretic process in which the lag period corresponds to a slow dimerization step of the enzyme induced by the substrate which could combine with the non-polymeric form of the enzyme. The Km for L-tyrosine of the polymeric enzyme was 0.15 mM.

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