geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

In vivo rat pancreatic acinar cell function during supramaximal stimulation with caerulein


, : In vivo rat pancreatic acinar cell function during supramaximal stimulation with caerulein. American Journal of Physiology 249(6 Pt 1): G702-G710

Infusion of a supramaximal dose of caerulein results in acute interstitial pancreatitis in rats. We report studies of in vivo pancreatic acinar cell function during the initial 3.5 h of supramaximal stimulation with caerulein (5 .mu.g .cntdot. kg-1 .cntdot. h-1). Amino acid ([3H]phenylalanine) uptake was not altered, and there was no change in the rate or extent of protein synthesis or in intracellular transport of in vivo pulse-labeled proteins from microsome to zymogen granule-enriched fractions. However, the discharge of labeled protein was markedly inhibited. Radioautographic studies indicated that the pulse-labeled proteins retained in the gland were not located extracellularly but had accumulated within acinar cells, with a preferential distribution at the cell apex (presumably in zymogen granules) and in large vacuoles that form within the cell during hyperstimulation. Supramaximal stimulation with caerulein also caused increasing amounts of amylase and labeled proteins to be recovered in the postmicrosomal fraction. These findings suggest that supramaximal stimulation causes digestive enzymes to become localized in organelles that are fragile and subject to disruption during tissue homogenization. These organelles may be the vacuoles noted in morphological studies and believed to represent immature condensing vacuoles and/or crinophagic vacuoles.

US$29.90

PMID: 2417493


Other references

Dahl, A.A.; Skarstein, J., 1996: When the need is great, patients should receive help. Tidsskrift for den Norske Laegeforening 116(18): 2213-2214

Ziehe, M.; Gregorius, H.R., 1988: Selection caused by self fertilization ii. ecological constraints on selfing advantage. The generally held view that increased self-fertilization should be advantageous in the absence of counteracting selective forces reducing viability or fertility is re-examined. It is pointed out that the models on which this view is based all imp...

Shindo, T.; Sadamasu, Y.; Suzuki, K.; Tanaka, Y.; Togawa, A.; Uematsu, Y., 2014: Method of quantitative analysis by HPLC and confirmation by LC-MS of sugar alcohols in foods. A reliable quantitative determination method of sugar alcohols, D-mannitol, xylitol and D-sorbitol, in food samples by HPLC, and a simple confirmation method by LC-MS were developed. Quantitative HPLC analysis was performed using a separation colu...

Lorz, C.; Eissner, C.; Lethmate, Jürgen.; Schneider, B., 2010: Spatial and temporal small-scale variability of nitrogen mobilization in a forest ecosystem with high N deposition in NW-Germany. For conifer stands in NW-Germany with high DIN load (23-35 kg N ha<sup>-1</sup> a<sup>-1</sup>) and a long history of nitrogen export the risk of N mobilization were investigated. Ammonium is the most mobilized N species, p...

Eshaghian, S.; Horwich, T.B.; Fonarow, G.C., 2006: Relation of loop diuretic dose to mortality in advanced heart failure. Although loop diuretics are widely used in heart failure (HF), their effect on outcomes has not been evaluated in large clinical trials. This study sought to determine the dose-dependent relation between loop diuretic use and HF prognosis. A cohor...

Pas'-Ko, Vi, 1976: Pathomorphological changes in the gastrointestinal tract of lambs infected by paratyphoid. Nauchnye trudy Stavropol'skii sel'skokhoziaistvennyi institut9(5): 65-67

Rifai A.; Small P.A.Jr; Teague P.O.; Ayoub E.M., 1979: Experimental immuno globulin a nephropathy. An animal model for Ig[immunoglobulin]A immune complex nephritis was developed. IgA immune complexes formed in vitro with an IgA anti-dinitrophenyl (DNP) derived from MOPC-315 plasmacytoma and dinitrophenylated bovine serum albumin (DNP-BSA) produ...

Van Boekel, M.A.J.S.; Crijns, C.L., 1994: Behaviour of the proteose-peptone fraction during renneting of milk. A study of what happens with proteose-peptone components (PP) during renneting of skim-milk showed that some PP components (probably PP5 and PP8, i.e. break-down products of beta-casein by plasma action) tend to associate with paracasein at the pH...

Marques, P.R.B. de O.; Nunes, G.S.; Queiroz, M.E.R. de; Orlanda, J.F.F.; Sousa, H.S. de; Santos, T.C.R. dos, 2002: Analysis of pesticides in environmental samples (water and sediments) of the Boa Esperanca barrage: Brazil - PI/MA: preliminary avaliation. Boa Esperanca barrage is a very important social and economical region for northeast of Brazil, and it needs continuous environmental monitoring. Water and sediments samples were collected to determine the most commonly used pesticides monocrotoph...

Henchoz Lecoanet, S.; Jeannin, P.; Aubry, J.P.; Graber, P.; Bradshaw, C.G.; Pochon, S.; Bonnefoy, J.Y., 1996: The Epstein-Barr virus-binding site on CD21 is involved in CD23 binding and interleukin-4-induced IgE and IgG4 production by human B cells. Human CD21 has previously been described as a receptor for the C3d,g and iC3b proteins of complement, as a receptor for the gp350/220 envelope glycoprotein of the Epstein-Barr virus (EBV) and also as a receptor for interferon-alpha (IFN-alpha). St...