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Indirect mutagen assay in human lymphocyte in the presence of a metabolic activation system


, : Indirect mutagen assay in human lymphocyte in the presence of a metabolic activation system. Journal of Hygiene, Epidemiology, Microbiology, and Immunology 30(4): 371-376

Chromosome aberrations in cultured human lymphocytes were examined after exposures to various concentrations (from 1 .times. 10-6 to 1 .times. 10-3 mol .cntdot. 1-1) of cyclophosphamide (CP) in the presence or absence of a metabolic activation system (S9 mix). With metabolic activation, increases in the frequency of aberrant cells (AB. C.) produced by CP were significant and dose-dependent. At a concentration of 5 .times. 10-4 mol .cntdot. 1-1, activated CP induced 29% AB. C. versus 6% AB. C. detected after exposures to CP without metabolic activation. The freshly prepared S9 mix did not virtually differ in its activation potency from the S9 mix stored for 3 weeks at -20.degree. C. CP preincubated for 100 min with S9 mix caused little or no increase in AB. C. frequency above the control level.

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PMID: 3805710


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