geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Induction of a fibrin gel investment an early event in line 10 hepato carcinoma growth mediated by tumor secreted products


, : Induction of a fibrin gel investment an early event in line 10 hepato carcinoma growth mediated by tumor secreted products. Journal of Immunology 122(1): 166-174

Within a few hours of implantation in the subcutaneous space of strain 2 guinea pigs syngeneic line 10 tumors became invested in a fibrin-gel. Tumors might induce and modulate this fibrin-gel by secreting molecules capable of activating the host's clotting and fibrinolytic systems. To test this possibility, line 10 tumor cells, and several non-neoplastic control cells, were cultured for 2 to 4 h in serum-free medium and the culture supernatants were assayed for their capacity to activate host inflammatory pathways. Four distinct mediator activities were recognized in line 10 tumor cell culture supernatants: a vascular permeability factor (VPF), a procoagulant (PC), a plasminogen activator (PA), and a macrophage-migration inhibitory activity (MIF). Nonmalignant control cells secreted none of these activities with the exception of rabbit kidney cells that not unexpectedly released a PA activity, presumably urokinase. Based on conditions of culture, physical and chemical properties, inhibitor studies, and kinetics of secretion, it is likely that 4 distinct molecules are responsible for these activities. The complexity of the culture medium was not critical but low pH was an important variable favoring PA secretion. Secretion of all mediators was inhibited by cold and that of VPF and PA by puromycin. Cytochalasin B and EDTA caused significant increases in PA secretion. The line 10 PA, like that from other sources, was irreversibly inactivated by diisopropyl fluorophosphate and is probably a serine esterase. The capacity to secrete mediators that activate important host inflammatory pathways provides tumors with a mechanism for stroma induction and may be an important factor in determining a tumor's histologic pattern and degree of malignancy.

US$29.90

PMID: 762415


Other references

Delgutte, B., 1979: Response of the auditory nerve to pure sounds having abrupt onset. Electrodiagnostic-Therapie 16(3): 139-139

Pebagallo, I.; Scuti, R., 1938: Defensive action of male hormone and vitamin E in infections. Several tests on male and female mice showed that injections of androsterone or vitamin E, or both combined, resulted in the survival of 34 to 55 per cent. of animals injected intraperitoneally with lethal doses of pneumococci. All the control mic...

JASPER.J.J.; McCANN.D.S.; MOSHER.R.E.; BOYLE.A.J., 1959: A surface tension equilibrium study of human serum in atherosclerosis. The rate of attainment of a static surface tension equilibrium by human blood serum was investigated. In general, serum from atherosclerotic individuals tends to reach a static surface tension value more rapidly than serum from a normal subject. F...

Bottu, Y.; L.M.Ire, J., 1963: Polymyositis in pediatrics. Two clinical cases of polymyositis are studied to define the general framework of polymyositis and the relation of this illness to the collagenosis. This illness is characterized by two principal syndromes: muscular, dominated by an a dynamism of...

Russell, R.L., 2007: Social communication impairments: pragmatics. Social communication or pragmatic impairments are characterized and illustrated as involving inappropriate or ineffective use of language and gesture in social contexts. Three clinical vignettes illustrate different pragmatic impairments and the w...

Al Hussein Alawamlh, O.; Al Hussein Al Awamlh, B.; Lee, R.K., 2017: Metabolic syndrome and voiding dysfunction. The metabolic syndrome (MetS) is an ever growing pandemic consisting of a constellation of abnormalities. Many hypotheses have been put forth to establish a link between this syndrome and voiding dysfunction. We created a layout of the possible, p...

Hara, S.; Hayasaka, S.; Mizuno, K., 1979: Partial purification and some properties of aryl sulfatases ec 3.1.6.1 a and b in the bovine neuro sensory retina. Lysosomal arylsulfatases A and B in the bovine neurosensory retina were studied biochemically using p-nitrocatechol sulfate as a substrate. Arylsulfatases A and B were partially purified to about 41-fold and 19-fold, respectively, from a crude ext...

Yan, C.; Zhao, S.; Jiang, S.; Xu, Z.; Huang, L.; Zheng, H.; Ling, J.; Wang, C.; Wu, W.; Hu, H.; Zhang, G.; Ye, Z.; Wang, H., 2006: Transcatheter closure of patent ductus arteriosus with severe pulmonary arterial hypertension in adults. Background: Surgical closure of patent ductus arteriosus (PDA) with severe pulmonary arterial hypertension in adults carries higher risk than in children.Objectives: To investigate the application of self-expandable occluders for transcatheter cl...

Dmitrieva, V.A.; Tsygankova, S.T., 1967: On blood compatibility in dogs during extracorporeal circulation. Eksperimental'naia Khirurgiia i Anesteziologiia 12(6): 84-84

Cool, S.M.; Hendrikz, J.K.; Wood, W.B., 1995: Microscopic age changes in the human occipital bone. The value of histological examination of the human occipital bone for estimation of age-at-death was assessed. Undecalcified sections of occipital bone from eighteen male Caucasian subjects between the ages of 21 and 70 years were prepared for ana...