geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Inhibition of acyl transferase ec 2.3.1.23 in lymphocytes by concanavalin a


, : Inhibition of acyl transferase ec 2.3.1.23 in lymphocytes by concanavalin a. Biochimica et Biophysica Acta 629(2): 305-316

The effects of concanavalin [Con] A and succinylated Con A on the transformation of mouse splenic lymphocytes and on early biochemical events in the transformation were compared. Lymphocyte transformation was biphasic with respect to Con A concentration, with optimal activation at .apprx. 1 .mu.g/ml. Activation by succinyl Con A was not biphasic over a range of lectin concentration of 1-16 .mu.g/ml. In intact lymphocytes cultured for 4 h, the enzyme Acyl-CoA:1-acylglycero-3-phosphocholine O-acyltransferase (EC 2.3.1.23) was not activated by Con A but was inhibited at all concentrations tested and was .apprx. 60% inhibited at 16 .mu.g Con A/ml. Succinyl Con A gave little or no inhibition at similar concentrations. Lymphocytes became committed to divide while their acyltransferase activities were markedly inhibited by Con A. The inhibition of acyltransferase by Con A was lifted by displacing the lectin from the cells by .alpha.-methylmannoside. Lowered enzyme activity was not caused by cell agglutination or by direct cross-linking of lectin receptors. Inhibition of acyltransferase was probably not due to indirect cross-linking via the cytoskeleton since colchicine did not reverse the inhibition. The inhibition of acyltransferase and the reduced stimulation of transformation by higher levels of Con A may be due to hydrophobic interaction of the lectin with the plasma membrane as shown by liposome aggregation studies.

US$29.90


Other references

Genta, R.M.; Weil, G.J., 1982: Antibodies to Strongyloides stercoralis larval surface antigens in chronic strongyloidiasis. Antibodies to intact rhabditiform and filariform larvae of Strongyloides stercoralis were measured by indirect immunofluorescence using quantitative fluorescence microscopy. Forty-four of 48 sera from infected patients (92 per cent) were positive...

MacMahon, B.; Yen, S., 1971: Influenza and neural-tube defects. Lancet 2(7718): 260-261

Aydın, E.; Özcan, Rşan.; Erdoğan, E.; Tekant, G., 2016: Comparison of the efficiencies of esophageal manometry, vector volume analysis and esophagus pH monitoring in the diagnosis of gastroesophageal reflux. In this study, we aimed to compare the superiorities of esophageal manometry, vector volume analysis and 24-hour pH meter studies in showing gastroesophageal reflux disease. The files of the patients who presented to pediatric surgery and pediatri...

Quan, H.B.; Li, T.Y.; Gao, Y.Y.; Chen, D.X., 2014: Clinical features of diabetes mellitus cases complicated by Burkholderia pseudomallei septicemia. The aim of this study was to analyze the clinical characteristics of diabetes mellitus patients with Burkholderia pseudomallei septicemia and evaluate strategies of diagnosis and treatment. The clinical characteristics, diagnosis, treatment, and p...

Nakasu, B.H.; Bassols, M. do C.; Feliciano, A.J., 1981: Temperate fruit breeding in Brazil. A brief survey of fruit breeding, chiefly of deciduous top fruit, in Brazil is given. New cvs are briefly described and include:- seven peaches for the fresh fruit market (Premier, BR-3, Princesa, Coral, BR-1, Vila Novo and Chiripa); nine peaches...

Nowak, K-E., 1975: Die Bedeutung des Zooplanktons fur de Stoffhaushalt des Schierensees. Archiv Hydrobiol, 752: 149-224

Bonetti, D.; Eccles, M.; Johnston, M.; Steen, N.; Grimshaw, J.; Baker, R.; Walker, A.; Pitts, N., 2005: Guiding the design and selection of interventions to influence the implementation of evidence-based practice: an experimental simulation of a complex intervention trial. A consistent finding in health services research is the report of uneven uptake of research findings. Implementation trials have a variable record of success in effectively influencing clinicians' behaviour. A more systematic approach may be...

Naotake, O.; Yasutarou, F.; Hirofumi, A.; Junichi, K.; Masayuki, O.; Iwane, S., 2006: Systematic genome function analysis of model bacteria. Tanpakushitsu Kakusan Koso. Protein, Nucleic Acid, Enzyme 50(16 Suppl): 2191-2197

Werner, S.C.; Block, R.J.; Mandl, R.H., 1960: Probable genetic basis for abnormal circulating iodoproteins Study of a family with several hypothyroid members with and without goiter. Unusual quantities of circulating iodoproteins, presumably peptide-linked iodo-amino acids, were identified in a 6-year-old hypothyroid boy with goiter, a 2-year-old hypothyroid sister without goiter, and a 59-year-old cousin who was a goitrous cr...

Lindner, J., 1956: Significance and fate of macromolecular substances in the human body, with special reference to dextran. Arztliche Forschung 10(6): I/275-I/298