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Inhibitor binding assay for angiotensin converting enzyme ec 3.4.15.1


, : Inhibitor binding assay for angiotensin converting enzyme ec 3.4.15.1. Clinical Chemistry 30(5): 696-700

A new principle for the determination of enzymes, applied to angiotensin-converting enzyme (ACE, EC 3.4.15.1) in human serum, is described. The enzyme inhibitor binding assay is based on specific binding of labeled inhibitor to the active center of the enzyme. Serum (10-15 .mu.l) is incubated with 125I-labeled ACE inhibitor (351A, a p-hydroxybenzamidine derivative of N-(1-carboxy-3-phenylpropyl)-L-lysyl-L-proline) at pH 7.0 at 37.degree. C for 2 h in a non-equilibrated system. Inhibitor bound to ACE is separated by adsorption to coated charcoal, the radioactivity remaining in the supernate is counted, and the ACE value is calculated from a standard curve. Sensitivity for ACE in serum is 200 U/l corresponding to 5.0 ng of ACE purified from human lung. The coefficient of variation was 3.9% within assay and 6.4% between assays for normal ACE activities. Correlation with a comparison spectrophotometric method for ACE assay was excellent (r = 0.98) in 59 samples from healthy subjects and from patients with various diseases including active sarcoidosis. The novel assay principle presented here is simple and specific and can be extended to use with various biological fluids and tissues and to other enzymes as well.

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Other references

Lippman, A.; Messing, K.; Mayer, F., 1990: Is genome mapping the way to improve Canadians' health?. Canadian Journal of Public Health 81(5): 397-398

Brown, C.R.; Strickler, S.A.; Moore, A.T.; Knutie, S.A.; Padhi, A.; Brown, M.Bomberger.; Young, G.R.; O'Brien, V.A.; Foster, J.E.; Komar, N., 2010: Winter ecology of Buggy Creek virus (Togaviridae, Alphavirus) in the Central Great Plains. A largely unanswered question in the study of arboviruses is the extent to which virus can overwinter in adult vectors during the cold winter months and resume the transmission cycle in summer. Buggy Creek virus (BCRV; Togaviridae, Alphavirus) is...

Szczepanski, R., 1972: Macrophotographic documentation of fungal cultures in the mycological laboratory on the example of the Tinea imbricata pathogen. Application possibilities and technical problems. I. Review and methods. Mykosen 15(8): 339-342

Kawashima, H.; Nomura, A.; Ohue, M.; Kato, H.; Kuwajima, J.; Furutani, Y.; Hosoki, K., 1997: A novel human beta-3-adrenoceptor agonist AD-9677. Diabetologia 40(SUPPL 1): A374

Haeseler C.W.; Saunders M.C.; Travis J.L.; Miller B.N., 1988: Expert systems for viticulture. American Journal of Enology & Viticulture 39(1): 101-102

Bower, David R., 1965: Precommericial thinnings accelerate diameter growth of loblolly pine. Heavy precommerical thinnings tripled diameter growth in dense 15-year-old stands of loblolly pine in the Ouachita Mountains of Arkansas. They also accelerated basal area growth, but slowed self-pruning.

Braun, T., 1990: Inhibition of the soluble form of testis adenylate cyclase by catechol estrogens and other catechols. The soluble form of rat germ cell adenylate cyclase was inhibited by compounds with a catechol moiety. Among the naturally occurring catechols tested, catechol estrogens were the most potent inhibitors. Catechol estrogens at 2-6 microM inhibited e...

Higuchi, M., 1986: Clinical significance and tentative definition of sinusoidal fetal heart rate pattern. Nihon Sanka Fujinka Gakkai Zasshi 38(6): 979-982

Carmo, K.Browning.; Barr, P., 2005: Drug treatment of neonatal seizures by neonatologists and paediatric neurologists. To survey anti-epileptic drug (AED) treatment of early-onset neonatal seizures by neonatologists and paediatric neurologists. A self-administered questionnaire was posted to Australian and New Zealand neonatologists and paediatric neurologists. Pa...

Tsirul'nikov, M.S., 1965: Surgical treatment of uterine bleeding. Akusherstvo i Ginekologiia 41(4): 124-128