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Investigations with pigs in the field of nutrition physiology for the evaluation of modified proteins 2. determination of endogenous nitrogen in the contents of the small intestines with the nitrogen 15 labeled tracer method


, : Investigations with pigs in the field of nutrition physiology for the evaluation of modified proteins 2. determination of endogenous nitrogen in the contents of the small intestines with the nitrogen 15 labeled tracer method. Archiv fuer Tierernaehrung 31(10): 675-683

The endogenous N-quota in the chyme of the small intestines after providing modified proteins was determined with re-entrant cannulae at the end of the ileum in investigations with pigs (35-50 kg live wt) with the isotope dilution method. The N-pool of the test animals was labeled with 15N-glycine and 15N-leucine. The trichloroacetate-soluble fraction of the blood plasma served as criterion for the labeling level of the N-pool. There was a direct connection between N-intake and endogenous N in the chyme of the small intestines. With regard to N-intake, the dependence of endogenous N in the chyme of the small intestines on feed protein could be observed.

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