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Isolation and properties of dna polymerase from the extremal thermophilic bacteria thermus flavus


, : Isolation and properties of dna polymerase from the extremal thermophilic bacteria thermus flavus. Biokhimiya 46(9): 1576-1584

A DNA-polymerase from the extremal thermophyilic bacteria T. flavus was isolated. The 5-step purification procedure resulted in an electrophoretically homogeneous enzyme with MW of 66,000. The isolated enzyme is thermostable and has a temperature optimum on the DNA templates at 70.degree. C and that on RNA templates at 50.degree. C. The enzyme does not contain contaminant endo- and exonuclease activities. The maximal activity of the enzyme requires the presence of template, 4 deoxyribonucleoside triphosphates and monvalent and bivalent cations in the incubation mixture. The enzyme is highly active when activated DNA, poly(dA)-poly(dT), poly(dA)-oligo(dT)10 and poly(rA)-oligo(dT)10 are used as templates, moderately active on single-stranded and double-stranded DNAs and inactive on poly(rC)-oligo(dG)12-18 and native RNA molecules.

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