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Isolation and properties of dna polymerase from the extremal thermophilic bacteria thermus flavus

, : Isolation and properties of dna polymerase from the extremal thermophilic bacteria thermus flavus. Biokhimiya 46(9): 1576-1584

A DNA-polymerase from the extremal thermophyilic bacteria T. flavus was isolated. The 5-step purification procedure resulted in an electrophoretically homogeneous enzyme with MW of 66,000. The isolated enzyme is thermostable and has a temperature optimum on the DNA templates at C and that on RNA templates at C. The enzyme does not contain contaminant endo- and exonuclease activities. The maximal activity of the enzyme requires the presence of template, 4 deoxyribonucleoside triphosphates and monvalent and bivalent cations in the incubation mixture. The enzyme is highly active when activated DNA, poly(dA)-poly(dT), poly(dA)-oligo(dT)10 and poly(rA)-oligo(dT)10 are used as templates, moderately active on single-stranded and double-stranded DNAs and inactive on poly(rC)-oligo(dG)12-18 and native RNA molecules.


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Sodhi, H.S.; Orchard, R.C.; Agnish, N.D.; Varughese, P.V.; Kudchodkar, B.J., 1973: Separate pools for endogenous and exogenous cholesterol in rat intestines. Endogenous and exogenous cholesterol were pulse-labelled by intraperitoneal injection of mevalonate-2-3H and by gastric intubation of cholesterol-4-14C in rats with common bile and thoracic lymph duct fistulae. Some of the exogenous cholesterol to...

Brunetti, P.; Puxeddu, A.; Nenci, G., 1962: Congenital non-spherocytic hemolytic anemia due to deficiency of pyruvic-kinase. Haematologica 47: 505-576

Larose, E., 1958: The State Research Station for the Improvement of Crop Plants at Gembloux. A review of plant breeding at Gembloux from 1913 onwards is given. In particular, mention is made of improvements in wheat, spelt, oats, barley, leguminous forage plants, flax and tobacco.

Sarmiento, L.; Ayala, M.; Peña, S.; Rodríguez, Gín.; Fermín, Z.; Tapia, F.J., 2007: Phagocytosis of promastigotes and amastigotes of Leishmania mexicana by the FSDC dendritic cell line: ultrastructural study. Dendritic cells, which capture and present antigen to activate unprimed T cell, are found in most tissues. This work describes the ultrastructure of Leishmania mexicana phagocytosis by the fetal skin dendritic cell (FSDC) line, a Langerhans cell l...

Posford Duvivier Environment.; Marine Biological Surveys and Sea Sediments., 1997: Broad scale biological mapping of Plymouth Sound and Estuaries. English Nature Research Reports, 208 i-vi, 1-27

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Jha, S.K.; Sunil Kumar; Sanjeev Malaiya; Deepti Sharma, 2004: Balance sheet of N and K as influenced by integrated nutrient management practices on scented rice. A field experiment was conducted during the kharif season of 2000 in Chhattisgarh, Madhya Pradesh, India to investigate N and K soil balance in scented rice cv. Pusa Basmati-I under integrated nutrient management practices. The treatments included...

Takai, Y.; Kaibuchi, K.; Kikuchi, A.; Sasaki, T., 1992: Small G proteins and cytoskeletal control. Cell Structure & Function 17(6): 448

Kitamura, N., 1995: A chemiluminescence procedure for determination of the release of myeloperoxidase from activated human neutrophils. A chemiluminescence (CL) procedure was developed to determine the time course of the release of myeloperoxidase (MPO) from activated human neutrophils using two CL probes, luminol and MCLA. Luminol-dependent CL (LDCL) and MCLA-dependent CL (MDCL)...

Guan, D.; Tkatch, T.; Surmeier, D.J.; Armstrong, W.E.; Foehring, R.C., 2007: Kv2 subunits underlie slowly inactivating potassium current in rat neocortical pyramidal neurons. We determined the expression of Kv2 channel subunits in rat somatosensory and motor cortex and tested for the contributions of Kv2 subunits to slowly inactivating K+ currents in supragranular pyramidal neurons. Single cell RT-PCR showed that virtu...