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Isolation and properties of dna polymerase from the extreme thermophilic bacteria thermus ruber


, : Isolation and properties of dna polymerase from the extreme thermophilic bacteria thermus ruber. Biokhimiya 47(11): 1785-1791

A DNA-polymerase from the extreme thermophilic bacteria T. ruber was isolated. A 6-step purification procedure resulted in an electrophoretically homogeneous enzyme preparation with MW of 70,000. The isolated enzyme is thermostable and has a temperature optimum on DNA templates at 70.degree. C and on RNA templates at 50.degree. C. The enzyme does not contain contaminant endo- and exonuclease activities. The maximal activity of the enzyme requires the presence of template, 4 deoxyribonucleoside triphosphates, and monovalent and bivalent cations in the incubation mixture. The catalytic activity of the enyzme on various templates of DNA and RNA types was investigated. A comparative physicochemical study of this DNA-polymerase and an analogous enzyme isolated earlier from T. aquaticus YT-1 was carried out.

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PMID: 7150670


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