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Isolation and properties of dnase from oncorhynchus gorbuscha testes


, : Isolation and properties of dnase from oncorhynchus gorbuscha testes. Izvestiya Sibirskogo Otdeleniya Akademii Nauk SSSR Seriya Biologicheskikh Nauk (3): 112-115

DNAse has been isolated and purified from Oncorhynchus gorbuscha milt using extraction, precipitation from dyalized with organic solvent extract and chromatography. pH Optimum 4,7-4,8. The enzyme was activated with low concentrations of two-valent ions Mg2+, Ca2+. Anions PO43- increased and anions SO42- decreased the enzyme activity. The enzyme hydrolysed native DNA 2.5 times faster than the denaturated one. Endonucleas type of action has been found out. By its properties the isolated enzyme was similar to DNAse form Oncorhynchus tshawytscha testis.

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Olsen, H.; Poulsen, L.B.; Andersen, B.V.stbo; Hansen, S.; Hansen, M., 2002: Collecting bag for human body wastes having an improved discharge means. A collecting bag comprising a bag member formed by two film blanks with joined edges, an inlet opening being provided in one of the film blanks surrounded by connecting elements for connection of the bag to a body orifice. The bag is emptied throu...

Szende, B.; Lapis, K.; Pal, K.; Sipos, L.; Denes, L.; Kisfaludy, L., 1987: The effect of TP-3 (Arg-Lys-Asp), TP-4 (Arg-Lys-Asp-Val), and TP-5 on the metastatic capacity of intravenously injected Lewis lung tumor cells. The oligopeptides Arg-Lys-Asp (TP-3), Arg-Lys-Asp-Val (TP-4), and Arg-Lys-Asp-Val-Tyr (TP-5) considered as the active short fragments of thymopoietin were administered (lo mg/kg) to C57B1 mice 24 hours before the intravenous inoculation of Lewis l...

Beermann, D.H.; Liboff, M.; Wilson, D.B.; Hood, L.F., 1983: Effects of exogenous thyroxine and growth hormone on satellite cell and myonuclei populations in rapidly growing rat skeletal muscle. We have examined the independent and combined effects of thyroxine (T4) and bovine growth hormone (bGH) on postweaning growth, satellite cell proliferation and accumulation of myonuclei in extensor digitorum longus (EDL) muscles in rats. Daily sub...

Hasson Kenneth O., 1974: The Greendale and Newman synclines, northeast Tennessee, interpreted as primary depositional synclines. Abstracts with Programs - Geological Society of America 6(4): 362

Kaida, K.; Kusunoki, S., 2009: Guillain-Barré syndrome: update on immunobiology and treatment. Growing experimental and clinical data have shed light on the pathophysiology of Guillain-Barré syndrome (GBS) and have further promoted the development of novel therapeutic strategies for the disorder. Elevated titer of antiganglioside antibodie...

Imamura, T.; Friedman, S.A.; Gamble, S.; Tokita, Y.; Opalenik, S.R.; Thompson, J.A.; Maciag, T., 1995: Identification of the domain within fibroblast growth factor-1 responsible for heparin-dependence. While the prototype members of the fibroblast growth factor (FGF) family, FGF-1 and FGF-2 are structurally related, the structural differences between these polypeptides predict that they will ultimately exhibit different biological roles. Indeed,...

Florinskaya, A.A., 1979: The parasite fauna of fish in the river Angara basin. A study is reported of fish parasites in the Bratsk reservoir (8 to 10 years after its construction on the river Angara, USSR), in the river Angara at the projected site of the Ust-Ilim reservoir and in the first year of the reservoir being filled...

Luderschmidt C., 1985: Hormonal control of sebaceous gland function. Medizinische Klinik 80(22): 628-633

Ionescu Tirgoviste C.; Bajenaru O.; Zugravescu D.; Dorobantu R.; Aciu M.; Rudzinsky O.; Mincu I., 1985: The electric potentials of the jing distal points in diabetics with and without polyneuropathy. The distribution of electric potentials on the Jing distal points in patients with diabetes mellitus with and without neurologic complications. The potentials were recorded by a sensor electrode on the surface of these points in 32 patients with d...

Kayaalp, C., 2006: Prevention of biliary leaks by biliary drainage after hydatid liver surgery. Digestive Surgery 22(5): 380; Author Reply 380-380; Author Reply 380