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Isolation of species specific and stage specific adhesion promoting component by dis aggregation of intact sea urchin strongylocentrotus purpuratus embryo cells


, : Isolation of species specific and stage specific adhesion promoting component by dis aggregation of intact sea urchin strongylocentrotus purpuratus embryo cells. Experimental Cell Research 137(2): 472-476

The supernatant obtained from dissociation of S. purpuratus blastula embryos effectively promoted reaggregation of S. purpuratus blastula cells by more than 3-fold over that in the absence of supernatant. The aggregation enhancing effect was completely species-specific and developmental stage-specific. The supernatant enhanced aggregation of glutaraldehyde-fixed cells, suggesting that it acts at the cell surface. Trypsinization of blastula cells did not affect the ability of dissociation supernatant to promote reaggregation; trypsin treatment of dissociation supernatant completely destroyed reaggregation enhancing activity. The attributes of this system are several: future biochemical work should be informative because liters of active supernatant containing about 1 mg protein/ml can easily be obtained; all experiments are done in seawater, the natural medium of the cells, resulting in experimental manipulations that are not greatly different from the normal living conditions of the cells; the isolated adhesion-promoting component is species-specific and stage-specific and appears to act at the cell surface.

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