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Isolation of the nuclear gene encoding a subunit of the yeast saccharomyces cerevisiae mitochondrial rna polymerase


, : Isolation of the nuclear gene encoding a subunit of the yeast saccharomyces cerevisiae mitochondrial rna polymerase. Journal of Biological Chemistry 261(22): 10348-10351

Antisera directed against the purified 145,000-dalton subunit of the Saccharomyces cerevisiae mitochondrial RNA polymerase have been used to immunoscreen a library of yeast genomic inserts constructed in the fusion protein expression vector, .lambda.gt11. A 4- kilobase pair yeast DNA fragment inserted into one of the recombinant bacteriophages appears to contain most or all of the gene that encodes the 145,000-dalton subunit.

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