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Lack of metabolism of progesterone to 5 alpha products in dog and guinea pig testes compared with immature rat testes


, : Lack of metabolism of progesterone to 5 alpha products in dog and guinea pig testes compared with immature rat testes. Journal of Steroid Biochemistry 10(2): 173-178

Testicular homogenates from immature and adult dogs and guinea pigs and those from immature rats were incubated with [3H]-progesterone or [14C]-3.beta.-hydroxy-5-pregnen-20-one in the presence of cofactors. After incubation, radioactive products were separated and identified by column and paper chromatography with derivative formation and recrystallization to constant specific activity. All testes from dogs and guinea pigs of different ages converted significant amounts (up to 80%) of 3.beta.-hydroxy-5-pregnen-20-one and progesterone to 17-hydroxy-4-pregnene-3,20-dione, 4-androstene-3,17-dione and testosterone, but these testes converted very small amounts (less than 1% in dogs and less than 5% in guinea pigs) of these precursors to 5.alpha.-reduced metabolites of 4-ene-3-oxosteroids. In immature rat testes under the same incubation conditions, conversion of progesterone to 5.alpha.-reduced steroids reached 70%. In contrast to rats and mice, immature testes of dogs and guinea pigs do not form large amounts of 5.alpha.-reduced C19-steroids.

US$29.90

PMID: 513729


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