+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Lack of soluble carbonic anhydrase in aortic smooth muscle of the rabbit

, : Lack of soluble carbonic anhydrase in aortic smooth muscle of the rabbit. Pfluegers Archiv European Journal of Physiology 405(3): 234-236

Rabbit aorta was investigated for the occurrence of soluble, methazolamide-sensitive carbonic anhydrase (CA) by measuring electrometrically the rate of acidification of a weakly alkaline CO2 solution buffered with 12.5 mM veronal/HCl at C. For this purpose, aortae of 10 rabbits with the endothelium carefully preserved, were homogenized, centrifuged, and the supernatants pooled. The proteins were fractionated by FPLC, and tested for their CA activity. In control experiments, the pH change resulting from the spontaneous CO2 hydration was found to be -0.92 .+-. 0.01 pH units/min (mean .+-. SE, n = 10). Aliquots of the 30,000 dalton protein fraction corresponding to 100 mg of aortic tissue wet weight did not alter the hydration rate significantly (-0.94 .+-. 0.01 pH units/min, n = 10). Also, in the presence of 10-4 M of the CA inhibitor, methazolamide, these rates were not altered significantly (-0.94 .+-. 0.01 and -0.93 .+-. 0.01 pH units/min, respectively, n = 10). No CA activity was found in the other FPLC fractions, etiher. These results suggest that soluble CA is absent from the myocytes and the endothelium of the rabbit aorta.


PMID: 3934642

DOI: 10.1007/bf00582566

Other references

Zahir, Z.; Asghar, H.; Arshad, M., 2001: Cytokinin and its precursors for improving growth and yield of rice. Phytohormones are well known for their regulatory role in plant growth and development and cytokinins represent one of the five well-established classes of phytohormones. A field experiment was conducted to evaluate the effect of a synthetic cytok...

Meyer, U.; Sermann, H.; Buttner, C., 2002: Frankliniella occidentalis (Pergande, 1895) - biological control by using entomopathogenic fungi. Trials were conducted to investigate the behaviour of the entomopathogenic fungus, Verticillium lecanii, in the population of the western flower thrips, Frankliniella occidentalis. We have found an autodissemination-effect of V. lecanii in the F....

Miki, T.; Lee, Y.H.rng; Tandin, A.; Subbotin, V.; Goller, A.; Kovscek, A.; Fung, J.J.; Valdivia, L.A., 2001: Hamster-to-rat bone marrow xenotransplantation and humoral graft vs. host disease. Bone marrow transplantation (BMT) may induce tolerance across xenogeneic barriers. We have established a xenogeneic BMT model where hamster BM is transplanted into splenectomized LEW rat recipients resulting in high levels of engraftment. Unfortun...

Sundaram, S.; Eisenhuth, J.; Howard, G.; Brandwein, H., 2000: Methods for validating microbial removal performance of 01 micron rated sterilizing grade filters. Abstracts of the General Meeting of the American Society for Microbiology 100: 617

Contreras, R.J.; Studley, J.L., 1994: Amiloride alters lick rate responses to NaCl and KCl in rats. The role of cation channels on taste cell membranes to salt taste sensation was assessed in rats. We measured the number of licks during multiple 10-s presentations of NaCl and KCl concentrations (0.05, 0.09, 0.16, 0.28, 0.5 M) dissolved in either...

Li, Z.; Zhu, J., 1986: The principle of FEM Deformable Element Joint Block and application in agricultural machine design. Nung yeh chi hsieh hsueh pao = Transactions of the Chinese society of agricultural machinery: ) 87-98

Takabatake Akira, 1960: Occurrence of uranium in the Neogene sediments recently found in the inner zone of northeast Japan. Kozan Chishitsu = Mining Geology 10(40): 124-126

D.V.s, L.; van der Nest, M.A.; van der Merwe, N.A.; Myburg, A.A.; Wingfield, M.J.; Wingfield, B.D., 2011: Genetic analysis of growth, morphology and pathogenicity in the F(1) progeny of an interspecific cross between Fusarium circinatum and Fusarium subglutinans. Fusarium circinatum and Fusarium subglutinans are two distinct species in the Gibberella fujikuroi species complex. A genetic linkage map produced from an interspecific cross between these species was used to identify quantitative trait loci (QTLs...

Shilenok, V.N.; Shappo, G.M., 1985: Effect of surgical treatment of duodenal ulcer on immune status. Khirurgiia: 69-72

ZhangBingWen(etal), 2008: The influence of different concentrations of liquor fermented cabbage to the content of nitrite. Cabbage was used as raw material to study the influence of different concentrations of liquor fermented products on nitrite content, microbial count, pH and acid change. Addition of liquor in the fermentation of cabbage resulted in lower nitrite c...