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Lack of specific binding of labeled rhizobium japonicum lipopolysaccharides to wild soybean glycine soja roots


, : Lack of specific binding of labeled rhizobium japonicum lipopolysaccharides to wild soybean glycine soja roots. Plant & Soil 84(1): 115-120

Lipopolysaccharides (LPS) were extracted from 2 strains of R. japonicum (61A76NS and 311b110-I). The extracted LPS was purified by gel filtration column chromatography and the amount of 2-keto-3-deoxyoctonate (KDO) was determined. Column purified LPS from both strains were conjugated to rhodamine isothiocyanate on celite to examine binding of this purified, labeled surface component to aseptically grown G. soja (wild soybean) seedlings as a basis for symbiotic specificity using fluorescent microscopy. Rhodamine conjugated LPS from both strains of R. japonicum did not exhibit specific binding to wild soybean seedling roots.

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