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Lack of systematic correlation between the interferon mediated anti viral state and the levels of 2' 5' oligo adenylate synthetase and protein kinase in 3 different types of murine cells

, : Lack of systematic correlation between the interferon mediated anti viral state and the levels of 2' 5' oligo adenylate synthetase and protein kinase in 3 different types of murine cells. Journal of Interferon Research 1(2): 179-190

The levels of 2 ds[double-stranded]RNA-dependent enzyme activities, pppA(2'p5'A)n synthetase (2-5A synthetase) and protein kinase, were investigated in control and interferon[IFN]-treated murine cells: L-929, K/Balb and NIH/3T3. Treatment of these cells with IFN resulted in the establishment of the antiviral response and the development of anticellular effects. The latter effect was observed 3 days after IFN treatment. The levels of 2-5A synthetase and protein kinase in control and interferon-treated cells seemed to vary among cell types. In L929 cells, both the 2-5A synthetase and protein kinase were induced by IFN. Treatment of NIH/3T3 cells with IFN resulted in the induction of 2-5A synthetase in the absence of any enhanced protein kinase activity. This lack of protein kinase in IFN-treated NIH/3T3 cells was not due to the presence of high levels of protein phosphatases. K/Balb cells contained very high levels of 2-5A synthetase in the absence of any apparent resistance to virus infection. On treatment with IFN, the level of 2-5A synthetase in K/Balb cells remained constant while the protein kinase activity was enhanced severalfold. Control and IFN-treated K/Balb cells showed similar sensitivity to the action of exogenous 2-5A, suggesting that the 2-5A system (the 2-5A dependent nuclease and the phosphodiesterase which degrades 2-5A) was not altered on treatment with interferon. The significance of these results in relation to the mechanism of the antiviral action of IFN is discussed.


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Lin, X.; Little, R.D.niel, 1997: Intermolecular diyl trapping reactions with allene diylophiles. Stereo- and regioselective intermolecular diyl trapping reactions were observed in these, the first examples of cycloaddition using allene diylophiles.

Powell, J.M.; Hons, F.M., 1991: Sorghum stover removal effects on soil organic matter content, extractable nutrients and crop yield. The effects of 2 sorghum cultivars, stover removal and N fertilizer (given as NH4NO3) on soil organic matter (SOM) content, extractable nutrients and crop yields were investigated in a 4 year experiment, on a Vertisol, in Texas, USA. Results indic...

Anonymous, 1956: CASE RECORDS of the Massachusetts General Hospital: weekly clinicopathological exercises: case No. 42051. New England Journal of Medicine 254(5): 226-235

Reveal, J.; Jarvis, C.; Barrie, F., 1990: Proposal to conserve the name and type of Acalypha virginica L. (Euphorbiaceae). Taxon 39(2): 361-363

Tansey, M.R.; Brock, T.D., 1972: The upper temperature limit for eukaryotic organisms. An upper temperature limit near 60 degrees for eukaryotic organisms is documented by results of a systematic search for fungi able to grow at higher temperatures. Samples from hot springs, thermal soils, self-heating coal waste piles, and other na...

van Kammen, D.P.; van Kammen, W.B.; Mann, L.S.; Seppala, T.; Linnoila, M., 1986: Dopamine metabolism in the cerebrospinal fluid of drug-free schizophrenic patients with and without cortical atrophy. Cortical atrophy measured with computed tomography was observed in ten of 53 schizophrenic patients. Levels of homovanillic acid, dihydroxyphenyl acetic acid (DOPAC), conjugated DOPAC, and dopamine sulfate (DASO4) were measured in the cerebrospina...

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Herrmann, W., 1981: The toxin content of Gyromitra fastigiata and Gyromitra gigas. The gyromitrin content for G. esculenta was 1480 mg/kg and for G. fastigiata and G. gigas < 0.5 mg/kg.

Révai, S.; König, E., 1968: Incidence of ABO blood groups in patients with diabetes mellitus. Zeitschrift für Arztliche Fortbildung 62(19): 1087-1088