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Lack of tuberculin activity of synthetic peptides

, : Lack of tuberculin activity of synthetic peptides. Infection and Immunity 50(3): 614-619

We synthesized an octapeptide, H-Asp-Gly-Gly-Ser-Glu-Ser-Glu-Gly-OH, and a hexadecapeptide, H-Asp-Gly-Gly-Ser-Glu-Ser-Glu-Gly-Lys-Asn-Gly-Ser-Gln-Met-Arg-Leu-OH, which corresponded to amino acids 61 to 68 and 61 to 76, respectively, of the amino acid sequence of a crystalline protein reported to be tuberculin active. Authenticity and purity of the synthesized peptides were confirmed by high-pressure liquid chromatography, amino acid analysis, mass spectrometry, and protein sequencer analysis. Tuberculin activity of the synthesized peptides was examined in guinea pigs sensitized with Mycobacterium tuberculosis or Mycobacterium bovis BCG and in tuberculin-positive healthy humans. Neither the octa- nor the hexadecapeptide was as active as tuberculin skin-test antigen.


PMID: 4066024

Other references

Lubrich, Jürgen., 2002: Distance nursing education in Australia: anywhere at any time. Pflege Zeitschrift 55(8): 591-593

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Yuonan, E.A.; Abdel-Gawad, S.M.; Teleb, Z.A., 1985: Studies on some steroidal hormones ii. the effect of ethynylestradiol on the actual release of some enzymes of rat liver lysosomes in vitro. The rate of some lysosomal enzyme release was studied under the influence of varying concentrations of ethinyl-oestradiol. The release was recorded after 3 time intervals of 30, 60 and 120 minutes. The recorded release, direct effect and the actua...

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