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Lack of ultrasound effect on in vitro human lymphocyte sister chromatid exchange

, : Lack of ultrasound effect on in vitro human lymphocyte sister chromatid exchange. Ultrasound in Medicine & Biology 10(3): 309-314

The frequency of sister chromatid exchanges (SC) in human lymphocytes cultured in vitro was not affected by a 30 min exposure to 2.25 MHz focused ultrasound beam (from a clinical diagnostic unit with a pulse repetition rate of 100 Hz, a 1 .mu.s burst duration, and a 2-200 W/cm3 maximum intensity). A 30 s exposure to continuous wave 1 MHz 2 W/cm2 (SP) ultrasound from an experiment device lysed 10-15% of the lymphocytes; there was no increase in the survivors relative to unexposed controls. Treatment of lymphocytes with 0.033 .mu.g/ml mitomycin-C, a known SCE inducer, increased the frequency of SCE about 4 times above control levels.


PMID: 6464218

DOI: 10.1016/0301-5629(84)90165-0

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