+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

LaCrosse virus gene expression in mammalian and mosquito cells

, : LaCrosse virus gene expression in mammalian and mosquito cells. Virology 165(2): 539-548

LaCrosse virus infection of mammalian BHK cells is highly cytopathic, whereas that of mosquito C6/36 cells is asymptomatic and persistent. When the individual mRNAs and their genome segments are followed in parallel infections, cytopathic effects were found to correlate with the rate of synthesis, but not the accumulation, of the viral RNAs. The change from the acute to the persistent phase of the infection in C6/36 cells was found to take place at 24 hr p.i., at which time genome and N protein synthesis was severely reduced, even though mRNA levels remained high. When the persistent infection was followed for 72 days, the total amounts of genomes and their relative proportions were found to fluctuate greatly, whereas mRNA levels were either severely reduced or undetectable. DI genomes could not be detected during this time. The self-limiting nature of the mosquito cell infection appears to be due the translational control of N protein synthesis.


PMID: 2900578

DOI: 10.1016/0042-6822(88)90598-3

Other references

Hargreaves J.A.; Rakocy J.E.; Bailey D.S., 1991: Effects of diffused aeration and stocking density on growth feed conversion and production of florida red tilapia in cages. The effects of four levels of diffused aeration (0, 6, 12, and 24 hours/day) and two stocking densities (400 and 600 fish/m3) on the culture performance of caged Florida red tilapia were evaluated in 1 m3 cages in a 2 ha watershed pond on St. Croi...

de Vries, H.E.; Moor, A.C.; Blom-Roosemalen, M.C.; de Boer, A.G.; Breimer, D.D.; van Berkel, T.J.; Kuiper, J., 1994: Lymphocyte adhesion to brain capillary endothelial cells in vitro. The presence and upregulation of adhesion molecules on bovine brain endothelial cells (BBEC) were investigated. Monolayers of BBEC were incubated with lipopolysaccharide (LPS), interleukin-1 beta (rhIL-1 beta), and interleukin-6 (rhIL-6) to simula...

Erdmann, E.; Califf, R.; Gerstein, H.C.; Malmberg, K.; Ruilope, L.; Schwartz, G.G.; Wedel, H.; Volz, D.; Ditmarsch, M.; Svensson, A.; Bengus, M., 2015: Effects of the dual peroxisome proliferator-activated receptor activator aleglitazar in patients with Type 2 Diabetes mellitus or prediabetes. Insulin-resistant states, including type 2 diabetes (T2D) and prediabetes, are associated with elevated cardiovascular (CV) risk. Aleglitazar is a dual peroxisome proliferator-activated receptor α/γ agonist with favorable insulin-sensitizing and...

Gispen, M.Elske.C., 2014: A human rights view on access to controlled substances for medical purposes under the international drug control framework. The world is confronted with a major public health deficit caused by poor access to controlled essential medicines under the international drug control framework. This is affecting millions of patients on a daily basis and resulting in numerous hu...

Farleigh Karen L., 1996: The influence of overwash events on the hydrology and vegetation of a pimple on Parramore Island, Virginia Coast Reserve. Abstracts with Programs - Geological Society of America 28(3): 52

Kato, Y.; Kurisu, S.; Mitsuba, N.; Ishibashi, K.; Dohi, Y.; Nishioka, K.; Kihara, Y., 2012: Effects of hypokalemia and left ventricular hypertrophy on QT interval in patients with primary aldosteronism. International Journal of Cardiology 152(3): 380-381

Mele, M.; Morelli, S.; Fazzari, G.; Avolio, E.; Alò, R.; Piscioneri, A.; D.B.rtolo, L.; Facciolo, R.Maria.; Canonaco, M., 2016: Application of the Co-culture Membrane System Pointed to a Protective Role of Catestatin on Hippocampal Plus Hypothalamic Neurons Exposed to Oxygen and Glucose Deprivation. NlmCategory="UNASSIGNED">Depletion of oxygen and glucose even for brief periods is sufficient to cause cerebral ischemia, which is a predominant worldwide cause of motor deficits with the reduction of life quality and subsequently dea...

Zhang, X.C.; Qian, Y.L.; Dang, B.; Xiao, S.Q.; Zhu, Q.L., 1989: Diagnosis of toxoplasmosis in pigs by dot-ELISA. Using a mixed nitro- and aceto-cellulose membrane as a carrier, dot-ELISA for diagnosing toxoplasmosis in pigs was studied and compared with PPA-ELISA and IHAT. The Levy's method was modified by adding EDTA to the blocking, washing and diluen...

Wilson, P.; Watson, R.; Ralston, G.E., 1995: Supporting problem drug users: improving methadone maintenance in general practice. British Journal of General Practice 45(398): 454-455

Ma, X.; Zhou, W.; Zou, Q.; Ouyang, P., 2016: Structural elucidation of the impurities in Enzalutamide bulk drug and the development, validation of corresponding HPLC method. NlmCategory="UNASSIGNED">As the first approved androgen receptor(AR) signalling inhibitor, Enzalutamide was approved by the US Food and Drug Administration as an anticancer drug used to treat castration-resistant prostate cancer in 20...