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Lactase production in continuous culture by trichoderma reesei rut c 30


, : Lactase production in continuous culture by trichoderma reesei rut c 30. Biotechnology Letters 6(9): 593-596

T. reesei Rut-C30 produces extracellular lactase when grown on lactose medium. Maximum enzyme levels in continuous culture were observed at dilution rates (D) between 0.02 and 0.027 h-1. The enzyme productivity reached 27.3 U/l h at D = 0.027 h-1. Lactase synthesis appears to be inducible and subject to catabolite repression. Optimal growth temperature and pH for enyzme production were 28.degree. C and pH 5. Maximum enzyme activity was observed at 63.degree. C and pH 4.6. The apparent Km, based on the nitrophenyl-galactopyranoside assay, was estimated as 0.4 mM. The enyzme is suitable for lactose hydrolysis in acid whey.

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