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Lactate content and ph in muscle samples obtained after dynamic exercise


, : Lactate content and ph in muscle samples obtained after dynamic exercise. Pfluegers Archiv European Journal of Physiology 367(2): 143-149

Analyses were made on muscle samples taken from the lateral part of the quadriceps femoris muscle of man (lactate, pyruvate, and pH) on venous blood (lactate, pyruvate) and on capillary blood (pH). Samples were taken at rest, immediately after termination of dynamic exercise and during 20 min recovery from exhaustive dynamic exercise. Muscle pH decreased from 7.08 at rest to 6.60 at exhaustion. Decrease in muscle pH was linearly related to muscle content of lactate + pyruvate. The relationship was slightly different from what was obtained after isometric exercise and this difference was ascribed to acid-base exchange with the blood during dynamic exercise. Lactate content was highly elevated in muscle after exercise and the concentration was 2-3 times higher than in blood. Pyruvate content was, however, only slightly higher than that at rest. During recovery lactate content of muscle decreased exponentially with respect to time, whereas pyruvate content increased. The half-time of lactate decrease was 9.5 min. From the lactate dehydrogenase equilibrium relative values on NADH/NAD ratio could be calculated. NADH/NAD was highly increased after exercise and it did not return to the basal value after 20 min recovery.

US$19.90

DOI: 10.1007/bf00585150


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