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Lactate dehydrogenase c ec 1.1.1.27 messenger rna its isolation and in vitro translation


, : Lactate dehydrogenase c ec 1.1.1.27 messenger rna its isolation and in vitro translation. Biochemical and Biophysical Research Communications 102(1): 93-99

Lactate dehydrogenase-C (LCH-C) mRNA was purified from DBA/2 mouse testes and translated in vitro. First, the LDH-C synthesizing polysomes were isolated by double immunoprecipitation using specific anti-LDH-C and anti-horse immunoglobulin antibodies. Extraction of mRNA was made from the isolated polysomes using hot sodium dodecyl sulfate(SDS)-phenol method at alkaline pH. In a wheat germ cell-free translation system, the mRNA coded for a polypeptide chain that could be immunoprecipitated with specific anti LDH-C antibody and comigrated with authentic LDH-C in SDS-polyacrylamide gel electrophoresis.

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