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Lactate dehydrogenase composition of the rabbit retina and choroid after ischemia caused by ligature of the optic nerve


, : Lactate dehydrogenase composition of the rabbit retina and choroid after ischemia caused by ligature of the optic nerve. Ophthalmic Research 9(3): 201-204

LDH [lactate dehydrogenase] isoenzymes of rabbit intraocular tissues were separated by thin-layer isoelectric focusing and localized as discrete groups of multiple bands detected by tetrazolium staining. In the rabbit intraocular tissues the predominant isoenzymes were LDH 3, 4 and 5. The bands showed microheterogeneity and were composed of 2-6 subcomponents. After 2 days of ischemia the LDH isoenzymes showed a considerable increase of activity in retina, choroid and iris. The appearance of LDH subcomponents in choroid and iris, and the disappearance of these components in cornea after ischemia was observed.

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