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Lactate dehydrogenase ec from autotrophic and heterotrophic cells of the marine diatom cylindrotheca fusiformis

, : Lactate dehydrogenase ec from autotrophic and heterotrophic cells of the marine diatom cylindrotheca fusiformis. Canadian Journal of Microbiology 22(10): 1502-1508

Cultures of C. furisormis grown either autotrophically or heterotrophically on lactate contained significant amounts of NAD-dependent L(+)-lactate dehydrogenase (EC Polyacrylamide gel electrophoresis of crude enzyme extracts revealed a single band which was indistinguishable between autotrophic and heterotrophic cells. The Km for lactate of partially purified preparations was lower under heterotrophic conditions. The specific activity in crude extracts was higher under autotrophic than heterotrophic conditions; it dropped precipitously when autotrophic cells were transferred to the dark, increasing again only in the presence of lactate. Apparently this enzyme has at most only a minor role in the assimilation of lactate during heterotrophic growth on lactate.


Other references

Peltier, L.F., 1987: The lineage of sports medicine. Clinical Orthopaedics and Related Research: 4-12

Djawdan, M.; Bradley, T.J., 1995: Selection for stress resistance in Drosophila does not lead to the evolution of lower metabolic rate. American Zoologist 35(5): 97A

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Fallowfield, L., 1995: Can we improve the professional and personal fulfillment of doctors in cancer medicine?. British Journal of Cancer 71(6): 1132-1133

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Ali, M.; Rawat, C.R.; Dhar, S.N., 1980: Single supplemental irrigation to sorghum under dryland conditions of Bundelkhand. The conversion of alternate inter-row spaces into shallow furrows 1 month after sowing sorghum in the kharif [monsoon] season of 1977 (a yr of low rainfall) increased grain yield by 15% by increasing rain water retention. Yield was increased 15-25...

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Norton, RA.; Bonamo, PM.; Grierson, JD.; Shear, WA., 1988: Fossil mites from the Devonian of New York State. Unknown

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