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Lactate dehydrogenase ec in developing rat oral epithelium

, : Lactate dehydrogenase ec in developing rat oral epithelium. Journal of Histochemistry and Cytochemistry 32(1): 1-6

Freeze-dried sagittal, whole-body sections of 10-day-old rats were incubated for lactate dehydrogenase (LDH) using different media in the presence of the inhibitors urea and fluoropyruvate. Phenazine methosulfate (PMS) and menadione, which are regularly used in current histochemical media and are believed to promote the demonstration of LDH activity, were also added and shown to be insufficient for the demonstration of total LDH activity, and PMS even seemed to have an inhibitory effect on LDH activity in oral epithelium. Cumulated data from the different incubations show that the oral epithelium of the developing rats may contain 2 different types of LDH, one in the basal cells with possibly aerobic characteristics and another in the spinosum/granulosum cells with anaerobic characteristics.


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