geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Lactate dehydrogenase ec 1.1.1.27 isozymes from the wood louse porcellio laevis porcellionidae isopoda physical and chemical properties


, : Lactate dehydrogenase ec 1.1.1.27 isozymes from the wood louse porcellio laevis porcellionidae isopoda physical and chemical properties. Indian Journal of Biochemistry and Biophysics 16(5): 278-283

P. laevis lactate dehydrogenase [LDH] has a MW of 140,000 daltons. It shows maximum activity at 0.3 mg .beta.-NADH and 18.16 .times. 10-2 M pyruvate incubated in 0.1 M phosphate buffer (pH 6.5) at 35-37.degree. C for 3 min. Under these optimal conditions 87% LDH activity is found in the hemolymph and muscles, and 13% in the hepatopancreas and alimentary canal. The enzyme is activated by sodium citrate, sodium oxalate, sodium fluoride, lithium chloride, EDTA and urea. ATP concentrations greater than 0.25 mM inhibit the enzyme. The Hill coefficient for the reaction containing ATP was 2.03, indicating that the enzyme has at least 2 ATP binding sites which interact in a cooperative, allosteric manner. Three isozymes of LDH were extracted from the woodlouse tissue, and their properties are described. The nature of the LDH isozymes is discussed.

US$29.90


Other references

Phippard, D., 1977: Hemi field differences in visual perception in deaf and hearing subjects. Verbal and nonverbal stimuli were presented tachistoscopically to 2 groups of deaf subjects, differentiated on the basis of primary communication mode, and to hearing controls. Hearing subjects exhibited a right visual field superiority for the pe...

Pooja; Prasher, P.; Singh, P.; Pawar, K.; Vikramdeo, K.Somesh.; Mondal, N.; Komath, S.Sudha., 2015: Synthesis of amino acid appended indoles: appreciable anti-fungal activity and inhibition of ergosterol biosynthesis as their probable mode of action. Rationally designed compounds consisting of mono- and di-peptide appendages on bis-indole template were synthesized in appreciable yield. Some of these compounds exhibited significant antifungal activities against Candida albicans with their MIC80...

Buzzi, A.; Henry, J.; Rossi, D., 1957: Aneurysm of the abdominal aorta with rupture into the inferior vena cava. Prensa Medica Argentina 44(20): 1527-1532

Khalakdina M., 1984: Training of personnel to work with young children in india. Early Child Development & Care: 247-276

Bouriquet, G., E.Al., 1954: The vanilla plant and vanilla throughout the world. The genus Vanilla is unique among the immense numbers of orchids in containing species of economic, as distinct from horticultural, importance. In spite of the large-scale manufacture of vanillin, the essential flavouring matter of the vanilla pla...

García-Saldivia, M.; Ilarraza-Lomelí, H.; Myers, J.; Lara, J.; Bueno, L., 2016: Effect of physical training on the recovery of acute exercise, among patients with cardiovascular disease. Physical training programs (PTP) have shown several beneficial effects for patients with cardiovascular disease (CVD), particularly by increasing survival and quality of life. Physiological response during the effort and recovery phases of an exer...

Haber, M.; Zhou, L.; Murai, K.K., 2006: Cooperative astrocyte and dendritic spine dynamics at hippocampal excitatory synapses. Accumulating evidence is redefining the importance of neuron-glial interactions at synapses in the CNS. Astrocytes form "tripartite" complexes with presynaptic and postsynaptic structures and regulate synaptic transmission and plasticit...

Garza-González, M.T.; Alcalá-Rodríguez, M.M.; Pérez-Elizondo, R.; Cerino-Córdova, F.J.; Garcia-Reyes, R.B.; Loredo-Medrano, J.A.; Soto-Regalado, E., 2011: Artificial Neural Network for predicting biosorption of methylene blue by Spirulina sp. An artificial neural network (ANN) was used to predict the biosorption of methylene blue on Spirulina sp. biomass. Genetic and anneal algorithms were tested with different quantity of neurons at the hidden layers to determine the optimal neurons i...

Klima, K.; Boligowa, E., 2002: Agricultural and environmental conditions of potato cultivation in the region of Doy Jasielsko-Sanockie. An analysis was carried out on the share of potato in the total crop structure and environmental conditions for 3 groups of communes in the region of Doy Jasielsko-Sanockie, Poland. The area analysed showed conditions favourable for agricultural p...

Marino, A.; Logi, G.; Roncucci, P.; Sodini, M.L.; Fornaini, F., 1993: Plasmapheresis and high-dose immunoglobulins in the treatment of acute demyelinating polyneuropathy. The authors report two clinical cases of Guillain-Barré syndrome, treated with contemporaneous use of plasmapheresis and high dose gammaglobulin. An early diagnosis and immediate application of such a therapeutic association, are of paramount imp...