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Lactate dehydrogenase ec 1.1.1.27 isozymes in the spermatozoa of the domestic fowl gallus domesticus and turkey meleagris gallopavo


, : Lactate dehydrogenase ec 1.1.1.27 isozymes in the spermatozoa of the domestic fowl gallus domesticus and turkey meleagris gallopavo. Comparative Biochemistry and Physiology B 61(3): 433-438

Electrophoresis of extracts of turkey spermatozoa for lactate dehydrogenase [LDH EC 1.1.1.27] activity revealed the usual 5 tissue LDH (LDH-1 to LDH-5). The presence of LDH-X (the spermatozoan-specific isozyme) was not obvious. Only 1 band was present on electrophoresis of fowl spermatozoan extracts and it coincided with LDH-1 (heart type). Kinetic investigations, the use of inhibitors and the heat-stability test confirmed that the fowl spermatozoan LDH was probably LDH-1 and not LDH-X.

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