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Lactate dehydrogenase ec in amoeba acanthamoeba sp in different phases and conditions of culture

, : Lactate dehydrogenase ec in amoeba acanthamoeba sp in different phases and conditions of culture. Comparative Biochemistry and Physiology B 61(1): 13-20

The activity and kinetic changes of amoeba LDH [lactate dehydrogenase EC] in different phases and conditions of culture were investigated. LDH of the amoeba was specific against D(-)LDH irrespective of the hypoxic conditions created. In hypoxic conditions it was not possible to visualize the presence of another LDH isozyme of muscle type by kinetic or electrophoretic analysis. Changes in the Km value and the L:H ratio as well as the decrease of electrophoretic mobility of the LDH band indicated change in kinetic properties of the enzyme from an obviously heart type in oxygenated culture towards a muscle type LDH in strongly hypoxic conditions. The influence of factors producing either environmental or metabolic hypoxia on possible repression or induction of LDH in amoeba is discussed.


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