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Lactate dehydrogenase ec 1.1.1.28 in amoeba acanthamoeba sp in different phases and conditions of culture


, : Lactate dehydrogenase ec 1.1.1.28 in amoeba acanthamoeba sp in different phases and conditions of culture. Comparative Biochemistry and Physiology B 61(1): 13-20

The activity and kinetic changes of amoeba LDH [lactate dehydrogenase EC 1.1.1.28] in different phases and conditions of culture were investigated. LDH of the amoeba was specific against D(-)LDH irrespective of the hypoxic conditions created. In hypoxic conditions it was not possible to visualize the presence of another LDH isozyme of muscle type by kinetic or electrophoretic analysis. Changes in the Km value and the L:H ratio as well as the decrease of electrophoretic mobility of the LDH band indicated change in kinetic properties of the enzyme from an obviously heart type in oxygenated culture towards a muscle type LDH in strongly hypoxic conditions. The influence of factors producing either environmental or metabolic hypoxia on possible repression or induction of LDH in amoeba is discussed.

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Other references

Gordon, Mp, 1983: Reversal of plant tumor formation Agrobacterium tumefaciens, Agrobacterium rhizogenes. NATO advanced study institutes series Series A Life sciences1(61): 133-135

Pauvolid-Corrêa, A.; Morales, M.Alejandra.; Levis, S.; Figueiredo, L.Tadeu.Moraes.; Couto-Lima, D.; Campos, Z.; Nogueira, M.Furlan.; da Silva, E.Elias.; Nogueira, R.Maria.Ribeiro.; Schatzmayr, H.Gonçalves., 2012: Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal. Despite evidence of West Nile virus (WNV) activity in Colombia, Venezuela and Argentina, this virus has not been reported in most South American countries. In February 2009, we commenced an investigation for WNV in mosquitoes, horses and caimans f...

Ye, B.; Wang, W.; Liu, S., 2003: Transarterial intrahepatic xenotransplantation of microencapsulated newborn porcine islets--an experimental study of type 1 diabetes mellitus. To evaluate the physiologic features and probable side effects of transarterial intrahepatic xenotransplantation of microencapsulated newborn porcine islets. Six artificial diabetic canines were transplanted intrahepatically with 6-9 x 10(5) encap...

Egeler C.G.; Simon O.J., 1969: Orogenic evolution of the Betic zone (Betic cordilleras, Spain), with emphasis on the nappe structures. Four tectonic complexes, constituent structural units, pre-Alpine orogeny and regional metamorphism, several phases of Alpine orogeny, nappe and post-nappe movements, age and direction, crustal shortening, Alpine metamorphism under syn- and postki...

Caballero Deloya, M.; Castellanos Jarjuin, S., 1974: Select bibliography on publications relating to forest inventories in Mexico. A bibliography of 162 references, with subject and geographical indexes.

Ryder, JP., 1978: Sexing ring-billed gulls externally. Bird-Banding: 493: 218-222

Bhat, R.G.; Hubbard, J.C.; Smith, R.F.; Koike, S.T.; Subbarao, K.V., 2000: Prevalence of Verticillium wilt of pepper in California. Phytopathology 90(6 Supplement): S7, June

Barker P.F.; Dalziel I.W.D.; Dinkelman M.G.; Elliot D.H.; Gombos A.M.J.; Lonardi A.; Plafker G.; Tarney J.; Thompson R.W.; Tjalsma R.C.; von der Borch C.C.; Wise S.W.J., 1977: Site 326. Initial Reports of the Deep Sea Drilling Project 36(Ushuaia, Argentina to Rio de Janeiro, Brazil, April-May 1974): 19-25

Sciotti Maurizio; Pinzari Mario, 1976: Some observations of manganese mineralization in northern Latium. Bollettino della Societa Geologica Italiana 95(3-4): 745-758

Siegl, J., 1957: Evaluation of ability for athletic performance in children. Wiener Medizinische Wochenschrift 107(16): 309-314