geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Lactate dehydrogenase inhibitors in NADH preparations


, : Lactate dehydrogenase inhibitors in NADH preparations. Clinical Chemistry 23(9): 1581-1584

The presence of a new lactate dehydrogenase inhibitor on the trailing edge of the NADH peak from chromatography on diethylaminoethyl-celluose [Loshon et al., Clin. Chem., this issue] was verified. It was resolved from the NADH by high-performance liquid chromatography on muBondapak C18. When the new inhibitor was present in a reaction mixture to the extent that, of the initial 260-nm absorbance, about 5% was contributed by the inhibitor, the rate of NADH oxidation by lactate dehydrogenase decreased by 65%. The inhibitor absorbs at 260 and 340 nm, and is different from the Strandjord-Clayson inhibitor [J. Lab. Clin. Med. 67, 144 (1966)] by both types of chromatography. Because this new inhibitor has ultraviolet properties similar to those of NADH and chromatographs with the NADH on DEAE-cellulose, the high-performance liquid chromatographic method must be used to ensure its absence in preparations of NADH used for clinical assay.

US$29.90

PMID: 196783


Other references

Sakuma, K.; Yamaguchi, A., 2010: The functional role of calcineurin in hypertrophy, regeneration, and disorders of skeletal muscle. Skeletal muscle uses calcium as a second messenger to respond and adapt to environmental stimuli. Elevations in intracellular calcium levels activate calcineurin, a serine/threonine phosphatase, resulting in the expression of a set of genes involv...

O'Shaughnessy, M.M.; O'Regan, J.A.; Murray, F.E.; Connell, J.A.; Duffy, M.P.; Francis, V.M.; Dwyer, S.; Thornton, L.M.; Conlon, P.J., 2012: Re-infection following sustained virological response with a different hepatitis C virus genotype: implications for infection control policy. We report the case of a 45-year-old haemodialysis patient who achieved a sustained virological response (SVR) following pegylated interferon therapy for hepatitis C virus (HCV) genotype 2 infection. He was subsequently cohorted with other HCV-infe...

Stephen, S., 1984: Teachers as dental health educators. Odonto-Stomatologie Tropicale 7(4): 205-208

Almeida, L.M.; Werneck, G.L.; Cairncross, S.; Coeli, C.M.; Costa, M.C.; Coletty, P.E., 2001: The epidemiology of hepatitis A in Rio de Janeiro: environmental and domestic risk factors. A serological study of hepatitis A was carried out in low-income areas scheduled for a major sanitation programme in Rio de Janeiro, Brazil. Blood spots were collected by finger puncture and transported on filter paper, and total antibodies to hep...

Črnigoj, M.; Podlesek, Z.; Zorko, M.; Jerala, R.; Anderluh, G.; Ulrih, Nša.Poklar., 2013: Interactions of archaeal chromatin proteins Alba1 and Alba2 with nucleic acids. Architectural proteins have important roles in compacting and organising chromosomal DNA. There are two potential histone counterpart peptide sequences (Alba1 and Alba2) in the Aeropyrum pernix genome (APE1832.1 and APE1823). THESE TWO PEPTIDES WE...

Swanson, P.D., 1995: Diagnosis of inherited metabolic disorders affecting the nervous system. Knowledge of the molecular causes for genetic diseases that affect the nervous system is rapidly expanding. Especially striking has been the finding in several autosomal dominant neurodegenerative disorders that unstable expansions of trinucleotid...

Gritti, I.; Mainville, L.; Jones, B.E., 1993: Codistribution of GABA- with acetylcholine-synthesizing neurons in the basal forebrain of the rat. In recent years, GABAergic neurons have been identified in the basal forebrain where cholinergic cortically projecting neurons are located and known to be important in mechanisms of cortical activation. In the present study in the rat, the relatio...

Anonymous, 2016: Chronic Disease Inquiry Focus On Home Care. Australian Nursing & Midwifery Journal 23(11): 13-13

Okano, K.; Matsuo, K., 1996: Seasonal changes in uptake, distribution and redistribution of 15N-nitrogen in young tea (Camellia sinensis L.) plants. In order to study seasonal changes in N requirements of tea plants, young plants (1-year-old rooted cuttings) of cv. Yabukita were grown hydroponically in a glasshouse, and pulse-labelled with 15N during different months of the year (May 1992 to A...

Saffiotti O.; Mosca S.; D.C.sare D.; Esposito F., 1988: Iatrogenic upper digestive hemorrhage. Drug-induced upper gastrointestinal hemorrhage is responsible for a considerable proportion of cases of upper gastrointestinal bleeding observed in emergency wards. The authors report their experience in light of the most recent international lite...