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Lactate dehydrogenase iso enzyme 1 ec 1.1.1.27 with the centrifugal analyzer after immunochemical removal of other iso enzymes


, : Lactate dehydrogenase iso enzyme 1 ec 1.1.1.27 with the centrifugal analyzer after immunochemical removal of other iso enzymes. Clinical Chemistry 27(6): 922-923

Measurement of lactate dehydrogenase isoenzymes in the serum provide reliable information for the diagnosis of myocardial infarction. A centrifugal analyzer method for measuring the LD-1 [lactate dehydrogenase isoenzyme] isoenzyme of lactate dehydrogenase in human serum, after immunochemical precipitation of the other 4 isoenzymes, is described. Enzymic activity was measured kinetically at 30.degree. C with the pyruvate-to-lactate assay. The method for LD-1 was linear to 1000 U/l. The precision (CV) of the assay was 1.0-2.2% within-run and 3.1-4.5% day-to-day. The reference interval was 26-73 U/l (n =51), corresponding to 21-35% of total LD activity.

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