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Lactate dehydrogenase iso enzyme distribution in human eosinophils

, : Lactate dehydrogenase iso enzyme distribution in human eosinophils. International Archives of Allergy & Applied Immunology 71(3): 193-199

The lactate dehydrogenase (LDH) isoenzyme pattern was determined in human eosinophils isolated from the peripheral blood of healthy donors and patients with parasitic diseases. Almost equal LDH1 and LDH5 values appear to be a characteristic of the eosinophils obtained from healthy subjects. Eosinophilic granulocytes which were isolated from the peripheral blood of patients with eosinophilia due to parasitic infestations have reduced values of LDH5 accompanied by higher LDH1 compared with eosinophils from healthy donors. Centrifugation of cells through multiple discontinuous gradients of polyvinylpyrrolidone-coated silica particles made it possible to isolate distinct eosinophil subpopulations of differing densities which showed differences in cell surface markers, K-cell activity and LDH patterns. Analysis of the LDH isoenzyme patterns showed that EAG+ and EAC+ eosinophilic leukocytes are characterized by very low values of LDH5.


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