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Lactate dehydrogenase isolated from human liver mitochondria its purification and partial biochemical characterization


, : Lactate dehydrogenase isolated from human liver mitochondria its purification and partial biochemical characterization. Clinical Biochemistry 21(4): 231-238

We have demonstrated that lactae dehydrogenase is not solely a cytostolic enzyme by the isolation and purification of the enzyme from the mitochondria of human liver. Treatment of the mitochondria with digitonin reveals the LD activity to be associated with the inner membrane-inner matrix and the outer membrane. The mitochondrial LD consists of two fractions separated by ion exchange and affinity chromatography. The first mitochondrial fraction, LD-Mt1, with isoelectric points of 9.8, 9.6, and 4.8, has subunit components of 14500 and 34000 daltons. The second mitochondrial fraction, LD-Mt2, is similar to cytosolic LD-5 with respect to both isoelectric points and subunit molecular weight. The first mitochondrial fraction, LD-Mt1, has physical characteristics previously associated with the isoenzyme LD-6.

US$19.90

PMID: 3409525

DOI: 10.1016/s0009-9120(88)80006-7


Other references

Chmurzynska, A.; Szydlowski, M.; Stachowiak, M.; Stankiewicz, M.; Switonski, M., 2007: Association of a new SNP in promoter region of the porcine FABP3 gene with fatness traits in a polish synthetic line. Associations between FABP3 ( alternatively named H- FABP) gene polymorphisms and fatness traits were tested in two pig breeds ( Polish Large White and Polish Landrace) and one synthetic line - 990. Three known single nucleotide polymorphisms, det...

Cerne, R.; Randic, M., 1993: Modulation of NMDA and AMPA responses by cAMP-dependent protein kinase and protein kinase C in spinal dorsal horn neurons. Society for Neuroscience Abstracts 19(1-3): 1531

Blanc, E.; Caron, J.; Fant, C.; Monier, E., 2017: Is current irrigation sustainable in the United States? An integrated assessment of climate change impact on water resources and irrigated crop yields. While climate change impacts on crop yields has been extensively studied, estimating the impact of water shortages on irrigated crop yields is challenging because the water resources management system is complex. To investigate this issue, we inte...

Geshele, E.E.; Rezvetsov, V.N., 1976: Phytopathological evaluations of resistance to powdery mildew in breeding wheat and barley. Information is presented on the racial composition of Erysiphe graminis on wheat and barley in the Black Sea steppe of the Ukraine and on the degree of resistance shown by the varieties evaluated.

Johnson, S.; Schindel, G.M.; Veni, G., 2009: Tracing groundwater flowpaths in the Edwards Aquifer recharge zone, Panther Springs Creek basin, northern Bexar County, Texas USA. Proceedings of the International Congress of Speleology 15, . 3: 1538

Venditti, S.; D.S.efano, G.; D.M.uro, E., 2000: Telomere-based neo-Darwinian selection of yeast clonal subpopulations. In Saccharomyces cerevisiae, imbalance of the genes coding for the heterochromatin components Sir3p and histone H4 (namely, overdosage of SIR3 and lack of one of the two genes coding for H4) causes modifications in telomere length and telomere seq...

Lira, G.M.; Guerra, N.B.; Pessoa, D.C.N. de P., 1992: Nutritional and sensory properties of canned cowpeas (Vigna unguiculata). 2. Effect of storage. A batch of 180 cans of cowpeas (Vigna unguiculata) was stored at 22 degrees to 32 degrees C for 6 months. At days 0, 30, 90 and 180 of storage 26 cans were sampled at random. Although after 30 days lipid and after 90 days protein showed a signific...

Grimes, S.L., 2016: Ten Questions With …. Biomedical Instrumentation & Technology 50(1): 9-9

Nathan, H.A.; Friedman, W., 1962: Chlorpromazine affects permeability of resting cells of Tetrahymena pyriformis. Incubation of cell suspensions of Tetrahymena pyriformis with chlorpromazine increased the permeability of the cell membrane. This permeability change could be measured either biochemically by the increased entrance of a chelator or physically by...

Edvardsson, N.; Varnauskas, E., 1987: Clinical course, serum concentrations and elimination rate in a case of massive sotalol intoxication. A young woman had been on antiarrhythmic treatment with sotalol 80-160 mg daily for three years because of ventricular tachycardia. After a quarrel she ingested an overdose of sotalol, estimated to be 13-14 g, and was immediately brought to hospit...