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Lactate dehydrogenase isozymes in developing rat oral mucosa a comparative study of lactic acid dehydrogenase ec biochemistry and histochemistry

, : Lactate dehydrogenase isozymes in developing rat oral mucosa a comparative study of lactic acid dehydrogenase ec biochemistry and histochemistry. Journal of Histochemistry and Cytochemistry 32(9): 958-964

Histochemical lactic acid dehydrogenase (LDH) staining methods seem unable to demonstrate the total LDH activity in tissue sections. An analysis was made of LDH tissue staining methods applied on LDH zymograms. The menadione-mediated LDH staining of tissue sections can not possibly reflect true LDH activity. The addition of cyanide also slightly inhibited LDH activity. The cyanide inhibition was confirmed via LDH assay and was competitive in character. Cyanide and menadione should be replaced by agents suitable from both a histochemical and a biochemical point of view. The presence of LDH in oral epithelium was analyzed. Evidently LDH of the oral epithelium is basically anaerobic in character and located primarily in spinosum/granulosum layers and only sparsely in the basal layer.


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