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Lactate dehydrogenase of the horseshoe crabs and their hybrids

, : Lactate dehydrogenase of the horseshoe crabs and their hybrids. Zoological Science (Tokyo) 1(3): 421-426

The lactate dehydrogenase (LDH) of the horseshoe crabs and their hybrids was analyzed with specific enzyme staining on starch gels. The LDH from hepatopancreas tissues of Tachypleus tridentatus and Carcinoscorpius rotundicauda consisted of 3 isozymes and the T. gigas LDH from hepatopancreas tissue occurred in 1, 3 or 6 enzymic bands. The American horseshoe crab, Limulus polyphemus, had the 2 LDH triads. Each embryonic LDH of 3 Asian horseshoe crabs showed only 1 electrophoretic band and the LDH of interspecific hybrid embryos occurred in 3 electrophoretic bands, suggesting that each embryonic LDH molecule of the 3 Asian species was a homodimer and that each LDH of the hybrid embryos was composed of a paternal homodimer, a maternal homodimer and a hybrid heterodimer. Electrophoretic patterns of the LDH in the Asian horseshoe crabs and their hybrids support the dimer model that the LDH triad is genetically controlled by 2 loci and that individuals with the common phenotype (3 dimer bands) are homozygous at both loci. The T. gigas .female. .times. C. rotundicaudal .male. eggs were grown into the 1st-instar larvae for the 1st time since the 1st experimental hybridization had been carried out in 1977. The LDH of this unusual hybrid also consisted of a paternal homodimer, a maternal homodimer and a hybrid heterodimer.


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Diers, D., 1990: Learning the art and craft of nursing. American Journal of Nursing 90(1): 64-66

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Dahlke, E.E.; Truhlar, D.G., 2007: Electrostatically Embedded Many-Body Expansion for Large Systems, with Applications to Water Clusters. The use of background molecular charge to incorporate environmental effects on a molecule or active site is widely employed in quantum chemistry. In the present article we employ this practice in conjunction with many-body expansions. In particula...

Drescher, J., 1957: Hemagglutinin and antibody content determinations. II. Studies on a hemagglutination-inhibiting antibody, and description of a photometric method for antibody content determination. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. 1. Abt. Medizinisch-Hygienische Bakteriologie, Virusforschung und Parasitologie. Originale 169(7-8): 461-470

Nishihara, M., 1987: CT of the thymus--I. CT image of the normal thymus. Nihon Igaku Hoshasen Gakkai Zasshi. Nippon Acta Radiologica 47(6): 799-803

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Feldman, L., 1950: Diabetic gangrene; considerations on its interpretation and treatment. Revista de la Asociacion Medica Argentina 64(673-674): 129-131

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