+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Lactate dehydrogenase part 10 separation of human spermatozoa iso enzymes by extraction with detergents

, : Lactate dehydrogenase part 10 separation of human spermatozoa iso enzymes by extraction with detergents. Research in Experimental Medicine 171(3): 297-300

Proteins with known enzymatic properties are attractive molecules as possible agents for immunological contraception. A new technique for the selective isolation of LDH[lactate dehydrogenase]-X from human spermatozoa is described. Applying the detergents hyamine 2389 and triton X-100, LDH-X was extracted from the sperm surface membrane without the other LDH-isoenzymes. Ultrasonification of spermatozoa yielded all LDH-isoenzymes together. After cell disruption the separation of LDH-X and the isoenzymes was performed by disc electrophoresis. With lactate as substrate, tetrazolium staining identified all LDH-enzymes. The technique represents an attractive method for the selective isolation of LDH-X and opens further purification steps.


Other references

Khan, S.H.; Kumar, R., 2017: Trehalose induced conformational changes in the amyloid-β peptide. Alzheimer's disease is an irreversible and progressive brain disorder featured by the accumulation of Amyloid-β (Aβ) peptide, which forms insoluble assemblies that builds up into plaques resulting in cognitive decline and memory loss. The f...

Mertsch, H., 1985: Guidelines for cerebral (brachiocephalic) angiography. Radiologia Diagnostica 26(3): 381-388

Yu, D-Shui.; Xu, Z-Qun., 2010: EPCA-2 in the early diagnosis of prostate cancer. More and more clinical evidence has confirmed the limitations of the use of serum PSA in the screening, detection and treatment of prostate cancer, and scientists are continuously seeking for new biomarkers of the disease. The discovery of early p...

Gan A.C., 1979: Non a non b hepatitis a new syndrome reported in hemo dialysis patients. Kidney International: 217

Anonymous, 1998: The use of various control methods over several years against apple and fruit-peel tortricids; action against target and nontarget organisms. The cumulative effects on target and nontarget organisms are described of repeated applications of chlorpyrifos-methyl, phosalone, fenoxycarb, teflubenzuron and 2 granulosis virus preparations against Cydia pomonella and Adoxophyes orana on apples...

Page, M.G.; Ankoma Sey, V.; Coulson, W.F., 1989: Brain glutamate and c-aminobutyrate (GABA) metabolism in thiamin-deficient rats. The British Journal of Nutrition 62: 5-53

Serafini, T.E.; Maitland, S.B., 2013: Validating the Functions of Identity Scale: addressing methodological and conceptual matters. Various studies have examined the validity of the Functions of Identity Scale; the current study addressed gaps in previous works. Its three main goals were to revise the Control subscale and establish its construct validity, to assess the structu...

Akkiraju, H.; Bonor, J.; Nohe, A., 2016: CK2.1, a novel peptide, induces articular cartilage formation in vivo. NlmCategory="UNASSIGNED">Bone morphogenetic protein 2 regulates chondrogenesis and cartilage formation. However, it also induces chondrocyte hypertrophy and cartilage matrix degradation. We recently designed three peptides CK2.1, CK2....

Padovan, I.; Oresković, M., 1971: Treatment of stenoses of the larynx. Archiv für Klinische und Experimentelle Ohren- Nasen- und Kehlkopfheilkunde 199(1): 254-291

Aliev, G.; Miller, J.P.; Leifer, D.W.; Obrenovich, M.E.; Shenk, J.C.; Smith, M.A.; Lamanna, J.C.; Perry, G.; Lust, D.W.; Cohen, A.R., 2007: Ultrastructural analysis of a murine model of congenital hydrocephalus produced by overexpression of transforming growth factor-beta1 in the central nervous system. The purpose of this study was to elucidate using transmission electron microscopy (TEM) the ultrastructural changes that occur within the cortical gray matter of a novel reproducible model of congenital hydrocephalus in mice created to overexpress...