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Lactate dehydrogenases of the australian fresh water blackfish gadopsis marmoratus identification of iso enzymes and immunochemical comparison of blackfish lactic dehydrogenase m 4 with the homologous enzyme from proposed marine ancestors


, : Lactate dehydrogenases of the australian fresh water blackfish gadopsis marmoratus identification of iso enzymes and immunochemical comparison of blackfish lactic dehydrogenase m 4 with the homologous enzyme from proposed marine ancestors. Comparative Biochemistry & Physiology B 74(2): 307-314

The electrophoretic properties and tissue distributions of LDH [lactate dehydrogenase] M. H and C subunit isoenzymes were compared in blackfish (G. marmoratus), rock cod (Physiculus barbatus), ling (Genypterus blacodes) and blenny (Pictiblennius tasmanianus). Immunochemical comparison of the LDH M4 isoenzymes purified from these 4 fish show that the ordering of antigenic similarity relative to the blackfish enzyme is rock cod > ling > blenny. While the electrophoretic properties and tissue distributions of the LDH isoenzymes can be accommodated within current classification schemes proposed for these fish, the antigenic similarities among the LDH M4 isoenzymes are incompatible with suggested phylogenetic relationships.

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