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Lactate dependent protein synthesis in round spermatids from rat testes


, : Lactate dependent protein synthesis in round spermatids from rat testes. Development Growth & Differentiation 23(3): 255-262

Lactate (20 mM) markedly increased protein labeling of round spermatids (steps 1-8) from rat testes. The stimulatory effect of lactate on protein labeling was also observed to some degree in spermatocytes and late spermatids (steps 13-16), but not in Leydig cells and 7 day-old testis cell suspensions. In the lactate-treated spermatids, 51% of the labeled proteins were found in the water-solubic fraction (the 105,000 .times. g 1 h supernatant); only 21%, in the control cells. The labeled proteins did not break down for at least 90 min in the presence of lactate. Actinomycin D (20 .mu.g/ml) had no effect on [3H]leucine incorporation into the water-soluble proteins of spermatids, while labeling of the water-insoluble proteins (the 105,000 .times. g 1 h pellet) was decreased by 23%. Apparently, the round spermatids might be the most susceptible for the lactate-induced stimulation of protein synthesis among various types of testicular cells, and that lactate increases the synthesis of water-soluble proteins which may be regulated in the translational level of protein synthesis.

US$19.90

DOI: 10.1111/j.1440-169x.1981.00255.x


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