+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Lipid staining for the electron microscope: a new method

, : Lipid staining for the electron microscope: a new method. Journal of Cell Science 19(3): 425-437

Tissues fixed in osmium tetroxide or in combined osmium and glutaraldehyde (Hinde), embedded in Spurr's medium, cut at 0.5-l .mu.m and mounted in Farrant's gum medium containing ethyl gallate, show good staining of lipid-containing structures (droplets of triglyceride, membranes, mitochondria, etc.) in the light microscope. Such preparations show moderate contrast in the electron microscope without further staining. But a specific increase in contrast in lipid-rich structures is obtained by partition of the tissues, before embedding, in 70% ethanol saturated with the monoterpene hydrocarbon myrcene, with or without the addition of 0.1% ethyl gallate, followed by osmium tetroxide. This method will visualize both saturated and unsaturated lipids including waxes.


PMID: 54360

Other references

Liss, C.; Fekete, M.J.; Hasina, R.; Lam, C.D.emthuy; Lingen, M.W., 2001: Paracrine angiogenic loop between head-and-neck squamous-cell carcinomas and macrophages. Angiogenesis, an essential step in the development of neoplasia, is a complex process that involves the interaction of tumor cells with stromal cells. Tumor-associated macrophages (TAMs) can participate in the induction of tumor angiogenesis and a...

Dal Forno, G.Ogliari.; Kist, L.Wilges.; de Azevedo, M.Barbieri.; Fritsch, R.Seemann.; Pereira, T.Carneiro.Brandão.; Britto, R.Socoowski.; Guterres, Sílvia.Stanisçuaski.; Külkamp-Guerreiro, I.Clemes.; Bonan, C.Denise.; Monserrat, Jé.María.; Bogo, Mício.Reis., 2014: Intraperitoneal exposure to nano/microparticles of fullerene (C₆₀) increases acetylcholinesterase activity and lipid peroxidation in adult zebrafish (Danio rerio) brain. Even though technologies involving nano/microparticles have great potential, it is crucial to determine possible toxicity of these technological products before extensive use. Fullerenes C60 are nanomaterials with unique physicochemical and biolog...

Harrington, T.D.; Tran, V.N.; Mohamed, A.; Renslow, R.; Biria, S.; Orfe, L.; Call, D.R.; Beyenal, H., 2016: The mechanism of neutral red-mediated microbial electrosynthesis in Escherichia coli: menaquinone reduction. The aim of this work was to elucidate the mechanism of mediated microbial electrosynthesis via neutral red from an electrode to fermenting Escherichia coli cultures in a bioelectrochemical system. Chemical reduction of NAD(+) by reduced neutral re...

Vaglio, N., 1950: Mechanism of extra-vitamin action of massive doses of vitamin D; clinical research in obstetrics and gynecology and experimental research. Archivio di Ostetricia E Ginecologia 55(3): 238-250

Maslakova, NI., 1975: The development and changes of planktonic Foraminifera on the boundary between the Mesozoic and Caenozoic.. Byulletin Moskovskogo Obshchestva Ispytatelei Prirody (Geol): 504: 145-146

Enoki Y., 1985: Isolation of alpha and non alpha chains from human adult canine pig and guinea pig hemoglobins and their physicochemical properties. Journal Of The Physiological Society Of Japan9: 367

Ma, Y., 2013: On Inference for Kendall's τ within a Longitudinal Data Setting. Kendall's τ is a non-parametric measure of correlation based on ranks and is used in a wide range of research disciplines. Although methods are available for making inference about Kendall's τ, none has been extended to modeling multip...

Pokrovsky V.V.; Eramova I.; Savchenko I.; Kuznetsova I., 1991: Hiv transmission in the ussr. Istituto Superiore Di Sanita Vii International Conference on Aids: Science Challenging Aids; Florence, Italy, June 16-21, 464p (Vol 1); 460p (Vol 2) Istituto Superiore Di Sanita: Rome, Italy Paper : 310b

Craenen, K.; Coosemans, J.; Ortiz, R., 1997: The role of stomatal traits and epicuticular wax in resistance to Mycosphaerella fijiensis in banana and plantain (Musa spp.). Twenty synthetic Musa hybrids of various ploidy, exhibiting a range of resistant and susceptible responses to the black sigatoka disease, were used to investigate the role of stomatal density, stomatal length and the thickness of epicuticular wax...

Jensen, P.E.; Kristensen, M.; Hoff, T.; Lehmbeck, J.; Stummann, B.M.; Henningsen, K.W., 1992: Identification of a single-copy gene encoding a type I chlorophyll a/b-binding protein in Arabidopsis thaliana. cDNA and genomic clones were isolated and sequenced from Arabidopsis thaliana which specify a 241 residue protein with 84% sequence identity to a photosystem I type I chlorophyll a/b-binding (CAB) protein from tomato. The open reading frame is int...