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Mechanism and methods of eliminating induced luminescence in immuno fluorescence


, : Mechanism and methods of eliminating induced luminescence in immuno fluorescence. Zhurnal Mikrobiologii Epidemiologii i Immunobiologii (2): 53-57

The causes of the appearance of induced fluorescence are discussed. The classification of its different forms is proposed. A hypothesis on the role of cytophilic properties of fluorescent antibodies in the mechanism of induced nonspecific fluorescence is proposed. This hypothesis was experimentally confirmed by using the Fab fragments of fluorescent antibodies to Rickettsia prowazekii and Rickettsia sibirica. Conclusions were made concerning the good prospects and advisability of using the Fab fragments of antibodies as an immunofluorescent reagent; the Fab fragments of pure antibodies may be considered an ideal variant of such a reagent.

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