+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Metabolism of parathyroid hormone by fetal rat calvaria

, : Metabolism of parathyroid hormone by fetal rat calvaria. Endocrinology 104(2): 510-516

These studies examine the metabolism of highly purified bovine parathyroid hormone [bPTH-(1--84)] by fetal rat calvaria. Enzymatically dispersed bone cells and intact (minced) calvaria were incubated with bPTH-(1--84) and the incubation medium was analyzed for degradation of PTH by polyacrylamide gel electrophoresis. Eluates of gel slices were assayed for immunoreactive PTH (iPTH) in carboxy- and amino-terminal RIAs. Both bone preparations metabolized bPTH-(1--84). The intact hormone progessively decreased with time and carboxy-terminal iPTH fragments were evident by 5 min of incubation. In the isolated cell preparations, intact hormone was completely degraded at submaximal doses of PTH (5 X 10(-9) M), as assessed by cAMP production. Degradation was incomplete in intact calvarial preparations at all doses studied. Intact calvaria were less sensitive to PTH with regard to cAMP production. No amino-terminal fragments were detected in the medium with either cell preparation. Oxidized (biologically inactive) bPTH-(1--84) was not metabolized in these systems. These findings contrast with studies in liver and kidney preparations, where oxidized bPTH has been shown to be degraded. These findings contrast with studies in liver and kidney preparations, where oxidized bPTH has been shown to be degraded. These data suggest that biological activity may be necessary for the metabolism of intact bPTH-(1--84) by bone cells and that skeletal tissue may contribute to the immunoheterogeneity of circulating PTH in the rat.


PMID: 221182

DOI: 10.1210/endo-104-2-510

Other references

Abraham, B.T.; Anirudhan, T.S., 2001: Effect of NTA and pH on lead(II) adsorption by the hydrous oxides of Mn, Fe, and Al. The object of this work was to study the influence of nitrilotriacetic acid (NTA) on Pb(II) adsorption by different hydrous oxide gels (MnOOH, FeOOH and AlOOH) at different pH levels. The surface charge density as a function of pH in aqueous solut...

Alon U.; Kodroff M.B.; Chan J.C.M., 1982: Type 4 renal tubular acidosis following renal vein thrombosis. Pediatric Research 16(4 PART 2): 317A

Millward, G.E.; Jha, A.N.; Minier, C.; Pope, N.D.; Langston, W.J., 2015: The English Channel and its catchments: Status and responses to contaminants. Marine Pollution Bulletin 95(2): 523-528

Laine, D.Landtsheer, G.Libe, T., 1953: Critical study of the direct surgical treatment of intracranial aneurysms of the carotid and its branches; two successive series of 14 surgical aneurysms. Revue Neurologique 89(6): 566-574

Jeon, W.Seon.; Park, C.Young., 2015: Antiobesity pharmacotherapy for patients with type 2 diabetes: focus on long-term management. Type 2 diabetes and obesity have a complex relationship; obesity is linked to insulin resistance, the precursor to type 2 diabetes. The management of obesity is an important method to delay onset of diabetes and improve the glycemic durability of...

Jiang, Y.; Peng, Q.; Geng, H.; Ma, H.; Shuai, Z., 2014: Negative isotope effect for charge transport in acenes and derivatives--a theoretical conclusion. The isotope effect (IE) on charge transport in polyacenes was proposed in 1970 to judge the transport mechanism. However, there had not been a definitive answer for more than 40 years as to whether such an IE is positive or negative, both theoreti...

Patrick, S.J.; Snelling, L.K.; Ment, L.R., 1993: Infantile chorea following abrupt withdrawal of diazepam and pentobarbital therapy. The acute onset of chorea was seen in a ten week-old male following abrupt cessation of a seven-day course of sedation with diazepam and pentobarbital. These movements slowly resolved over a six-day period and the infant was without neurologic seq...

Falk, M.; Sultana, R.; Swann, M.J.; Mount, A.R.; Freeman, N.J., 2016: Nanoband array electrode as a platform for high sensitivity enzyme-based glucose biosensing. We describe a novel glucose biosensor based on a nanoband array electrode design, manufactured using standard semiconductor processing techniques, and bio-modified with glucose oxidase immobilized at the nanoband electrode surface. The nanoband ar...

Lysholm, A.S.; Shah, N.; Lebien, T.W., 1999: Characterization of apoptosis induced via bone marrow stromal cell-deprivation of the human pre-B all cell line BLIN-2. Blood 94(10 SUPPL 1 PART 2): 158b, Nov 15

Chen, Q.Y., 1982: Preliminary study on Platypria melli Clhm. Preliminary studies were carried out in Jiangxi Province, China, on the biology of Platypria melli Uhm. on Paliurus ramosissmus. This appears to be the first record of the pest in the country. The egg, larva, pupa and adult are described. There wa...