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Methylation of high molecular weight rna

, : Methylation of high molecular weight rna. Lietuvos TSR Mokslu Akademijos Darbai Serija C Biologijos Mokslai (1): 129-138

Incorporation of methyl groups of methionine into high-MW, primarily ribosomal RNA extracted from hepatic cells of healthy rats, tumor-bearing rats and from Zajdela ascites hepatoma cells was studied. Two i.p. doses 4 h apart of L-[methyl-14C]methionine (25 .mu.Ci/100 g wt) to healthy rats and rats with Zajdela ascites hepatoma led to 2-fold increase in incorporation of 14C-methyl groups into high-MW RNA of the ver in tumor-bearing rats and in Zajdela ascites hepatoma cells to a 5 fold increase in comparison to controls. The nucleotide composition of high-MW RNA of the liver in healthy rats, tumor-bearing rats and in Zajdela ascites hepatoma cells was practically identical. The incorporation of methyl groups of L-[methyl-14C]methionine into uracil of the 3 types of cells was almost identical, while the methylation of cytosine, adenine and guanine in the liver of tumor-bearing rats and in Zajdela ascites hepatoma cells was 2 and 6 times greater, respectively, than in the liver of healthy rats. Approximately 73-77% of the methyl groups found in the high-MW RNA of healthy rat liver cells and in Zajdela ascites hepatoma cells were found in ribose, and the remaining 23-27% in bases. In liver RNA of tumor-bearing rats 48% of the methyl groups were found in the ribose and 52% in the bases.


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