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Methylation of high molecular weight rna


, : Methylation of high molecular weight rna. Lietuvos TSR Mokslu Akademijos Darbai Serija C Biologijos Mokslai (1): 129-138

Incorporation of methyl groups of methionine into high-MW, primarily ribosomal RNA extracted from hepatic cells of healthy rats, tumor-bearing rats and from Zajdela ascites hepatoma cells was studied. Two i.p. doses 4 h apart of L-[methyl-14C]methionine (25 .mu.Ci/100 g wt) to healthy rats and rats with Zajdela ascites hepatoma led to 2-fold increase in incorporation of 14C-methyl groups into high-MW RNA of the ver in tumor-bearing rats and in Zajdela ascites hepatoma cells to a 5 fold increase in comparison to controls. The nucleotide composition of high-MW RNA of the liver in healthy rats, tumor-bearing rats and in Zajdela ascites hepatoma cells was practically identical. The incorporation of methyl groups of L-[methyl-14C]methionine into uracil of the 3 types of cells was almost identical, while the methylation of cytosine, adenine and guanine in the liver of tumor-bearing rats and in Zajdela ascites hepatoma cells was 2 and 6 times greater, respectively, than in the liver of healthy rats. Approximately 73-77% of the methyl groups found in the high-MW RNA of healthy rat liver cells and in Zajdela ascites hepatoma cells were found in ribose, and the remaining 23-27% in bases. In liver RNA of tumor-bearing rats 48% of the methyl groups were found in the ribose and 52% in the bases.

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Other references

L.M.rra, C.; Morosi, G., 1966: Resuscitation of asphyxiated patients by means of expired air. Minerva Medica 57(5): 147-151

Strate, T.; Mann, O.; Standl, T.; Izbicki, J.R.; Knoefel, W.T., 2001: The potential of HBOC in acute pancreatitis. Anasthesiologie, Intensivmedizin, Notfallmedizin, Schmerztherapie 36 Suppl 2: S119-S120

Hobbs William Herbert, 1902: An instance of the action of the ice sheet upon slender projecting rock masses. American Journal of Science: ages 399-403. 1902.

Luke, M., 1981: Primary torsion of the major omentum as a cause of acute abdomen. Ugeskrift for Laeger 143(3): 122-123

Song, S.Y.; Suzuki, H.; Nakano, A.; Konishi, S., 2000: Tachykinins selectively enhance GABA-mediated transmission in the rat amygdala. Although tachykinins (TKs) are implicated in expression of emotional responses, we know little about the actions of TKs in the CNS sites associated with emotional behavior. Thus we explored the presence and functions of TK receptors in the amygdal...

Zucker, J.; Mascrès, C.; Charland, R., 1990: Oral manifestations of skin diseases. There exists a multitude of systemic diseases which exhibit consequential oral manifestations. Dermatologic disorders frequently involve intraoral and perioral tissues which dental practitioners should be familiar with. A compilation of disorders...

O'hara, C.M.; Chernoff, E.A.G., 1994: Growth factor modulation of injury-reactive ependymal cell proliferation and migration. Injury-reactive ependymal cells from regenerating axolotl spinal cord can be maintained in their mesenchymal outgrowth phase in culture (O'Hara et al., 1992). To address the ability of specific growth factors in stimulating or maintaining mig...

Krueger, F.; Groengroeft, A., 2004: The difficult assessment of heavy metal contamination of soils and plants in Elbe river floodplains. The investigations on total sums of heavy metals indicated that topsoils in the floodplains in the German part of the river Elbe are in part markedly enriched. The contamination exceeds the action values of the Federal Soil Protection and Contamin...

Ryser, E.T. .; Marth, E.H.; Buchanan, R.L.Reviewer, 1993: Food science and technology (Marcel Dekker, Inc.) (book review): V46: Listeria, listeriosis, and food safety. Food Technology 47: 7-8

Eckert, J., 2007: Historical aspects of echinococcosis - an ancient but still relevant zoonosis. The history of echinococcosis in Europe includes a period of over 2000 years. Already in antiquity metacestodes (hydatids) of Echinococcus granulosus, the causative agents of Cystic Echinococcosis (CE), were observed in animals and humans. Alveola...