+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Microtubules and desmin filaments during onset of heart hypertrophy in rat: a double immunoelectron microscope study

, : Microtubules and desmin filaments during onset of heart hypertrophy in rat: a double immunoelectron microscope study. Circulation Research 60(3): 327-336

The distribution of tubulin and desmin, the constituent proteins of microtubules and intermediate filaments, respectively, were studied in normal and hypertrophied rat myocardium by high-resolution immunofluorescence and immunoelectron microscopy. Cardiac hypertrophy was induced in 25-day-old rats by aortic stenosis. In the normal heart, double immunolabelling of ultrathin frozen sections of papillary muscle using gold-labelled probes for tubulin and desmin showed that microtubules ran primarily in a longitudinal direction through the intermyofibrillar spaces, perpendicularly to the desmin filaments. Microtubules were present near nuclei, mitochondria, and plasma membranes, while desmin filaments formed transverse connections between adjacent Z disks. No tubulin was observed near the intercalated disks, which were rich in desmin filaments. In hypertrophied hearts, myocytes exhibited the typical morphological features of developing hypertrophy. While there was little difference in the distribution of the microtubules around mitochrondria and at the plasma membrane, considerable increases were seen near the nuclei and along the myofibrils. Desmin labelling was distributed transversely as in the controls; however, sometimes it was longitudinally oriented either in the intermyofibrillar space linking 2 Z disks out of register or along digitations of the intercalated disks connecting neighboring desmosomes. The unique rearrangement of desmin and tubulin filaments in hypertrophied cardiac myocytes emphasizes their distinct role in myocyte organization. We suggest that, during the development of cardiac hypertrophy, desmin filaments are mainly involved in maintaining the myofibrils in register, whereas the degree of assembly of microtubules is correlated with the rate of protein synthesis and with myofibrillogenesis.


PMID: 2953507

DOI: 10.1161/01.RES.60.3.327

Other references

Vogen, K.W., 1996: Actinomycosis of the foot. Journal of the American Podiatric Medical Association 86(5): 238-240

González de Dios, J.; Pérez-Aytes, A., 1999: Genetics and dysmorphology in the context of pediatric subspecialties. Anales Espanoles de Pediatria 51(3): 222-229

Burrows, G.A., 1948: Leg brace. No. 2,456,046. Journal of the Patent Office Society. Patent Office Society 108: Unknown-Unknown

Arco, P.Rez, M.; Tomalino, D.; Muras, O., 1953: Chiari's syndrome; compression of the suprahepatic vein with participation of the upper third of the inferior vena cava by bronchial neoplasm. El Torax 2(1): 89-96

Noble, Glenn A., 1940: Trichoaina urechi n sp, an entozoic ciliate from the echiuroid worm, Urechis caupo. T. urechi, a peritriphous ciliate from the mid-gut of the annelid worm Urechis caupo, is descr.

Sun, P.; Zhang, F.; Chen, C.; Ren, C.; Bi, X-Wen.; Yang, H.; An, X.; Wang, F-Hua.; Jiang, W-Qi., 2016: Prognostic impact of body mass index stratified by smoking status in patients with esophageal squamous cell carcinoma. As smoking affects the body mass index (BMI) and causes the risk of esophageal squamous cell carcinoma (ESCC), the prognostic impact of BMI in ESCC could be stratified by smoking status. We investigated the true prognostic effect of BMI and its po...

Su, D.; Wellman, S., 1993: Amplification of H1 and H1e coding regions. FASEB Journal 7(3-4): A26

Sankaridurg, P.R.; Sharma, S.; Gopinathan, U.; Janakiraman, D.; Vuppala, N.; Sweeney, D.F.; Willcox, M.D.P.; Holden, B.A.; Rao, G.N., 1995: Hemophilus influenzae A causative organism in the pathogenesis of contact lens induced acute red eye. Investigative Ophthalmology & Visual Science 36(4): S630

Iliev, I., 1967: Physiological and biochemical changes in peach trees infected with Sphaerotheca pannosa var. persicae. III. The effect on the intensity of respiration and on the activity of some respiratory enzymes. The average respiratory intensity of the leaves increased by 63% and 40% in the varieties Marygold and Mayflower, respectively, when infected with powdery mildew. The increase was greater in the younger leaves and was not confined to the infected...

Frost, D., 1983: Relationships of the Baja California ground snakes, genus Sonora. S. bancroftae Klauber, 1943 and S. mosaueri Stickel, 1938 are placed in the synonymy of S. semiannulata Baird and Girard, 1853. The characters that have been used to separate these taxa (color pattern, numbers of ventrals and subcaudals, and dorsa...