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Mixed function oxidase activity in larvae of heliothis punctigera lepidoptera noctuidae


, : Mixed function oxidase activity in larvae of heliothis punctigera lepidoptera noctuidae. Journal of the Australian Entomological Society 23(4): 249-252

The O-demethylation of 4-nitroanisole was used to evaluate mixed-function oxidation (MFO) activity in last and penultimate instar larvae of H. punctiger. The specific activity of tissue of early 5th instar larvae in decreasing order were midgut, integument, fatbody, head capsule and whole larva. Specific activity of the midgut rose rapidly from the beginning of the instar and then returned to relatively low level when the insect stopped feeding. Fatbody specific activity increased until early in the last instar and remained at about this level until pupation. Specific activity of the integument was highest at the beginning and end of the instar and lowest in the middle. O-demethylase activity per larva revealed that although midgut activity was low late in the 5th instar, the insect still possessed relatively high levels of MFO's in the integument and particularly the fatbody.

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