+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Molecular dissection of human testicular germ cell differentiation with monoclonal antibodies

, : Molecular dissection of human testicular germ cell differentiation with monoclonal antibodies. Journal of Reproductive Immunology 12(3): 173-190

Monoclonal antibodies (MoAbs) were raised by immunizing mice with nucleated cells other than sperm (NCOS) obtained from semen of an oligospermic donor. These MoAbs recognized different germ cell differentiation antigens (GDA) which appear at various stages of spermatogenesis and/or spermiogenesis. The GDA were found to be differentially expressed on spermatocytes, spermatids and on the various sub-regions of human spermatozoa. Some of the MoAbs also recognized changes in the antigenic structure of the sperm during their post-testicular maturation. Using solubilized sperm products, MoAb GDA-J/F1 reacted with a 92 kDa band in Western immunoblotting. The importance of these antibodies in the study of germ differentiation together with their potential clinical uses in the investigation of infertility, autoimmunity and immunological contraception are discussed.


PMID: 3430485

DOI: 10.1016/0165-0378(87)90022-2

Other references

Bloom, J.M., 2001: Pravastatin and coronary heart disease. Lancet 357(9261): 1040-1040

Bennicelli, J.L.; Guerry, D., 1993: Cytokine production by cultured, human melanoma cells. Proceedings of the American Association for Cancer Research Annual Meeting 34(0): 449

Wireman, J.R.; Long, G.C., 2003: Communicating risk in diverse communities. Environmental risk communication involves the use of many tools and techniques to form and deliver effective messages. These messages need to inform affected communities about the potential risks and health effects resulting from exposures to envi...

Mäusle, E., 1973: Electron microscopic study on the role of the mesenchyma in regressive transformation by dexamethasone. Acta Endocrinologica. Supplementum 173: 25-25

Danilov, A.; Sadekov, R.; Vein, A., 2003: Gabapentin in migraine prophylaxis. Cephalalgia 23(7): 711-712, September

Ramamurthi, R.; Satyanarayana, K.; Selvarajan, V.R.; Swami, K.S., 1975: Distribution & synthesis of glycogen in the scorpion Heterometrus fulvipes (C. L. Koch). The quantitative distribution and synthesis of glycogen was studied in different tissues of females of the Indian scorpion Heterometrus fulvipes (Koch). Glycogen levels were found to be high in the hepatopancreas, intermediate in the ovary and low...

Kotwal, G.J.; Sarojini, H.; Chien, S., 2016: Pivotal role of ATP in macrophages fast tracking wound repair and regeneration. Chronic wounds occurring during aging or diabetes pose a significant burden to patients. The classical four-phase wound healing process has a 3-6 day lag before granulation starts to appear and it requires an intermediate step of activation of res...

Neumeyer, G., 1953: Accident and bone tuberculosis. Zentralblatt für Chirurgie 78(37): 1590-1594

Wong, R.Y.; Saber, S.S.; Ma, I.; Roberts, J.Mark., 2009: Using television shows to teach communication skills in internal medicine residency. To address evidence-based effective communication skills in the formal academic half day curriculum of our core internal medicine residency program, we designed and delivered an interactive session using excerpts taken from medically-themed televi...

Lam E., 1992: Changes in the ground beetle fauna of the gooi area during this century coleoptera carabidae. The groundbeetle fauna of the Gooi-area (southeastern part of the province of Noord-Holland, The Netherlands) was thoroughly investigated from 1982 to 1990. The area is a well known collecting ground for entomologist since the beginning of this ce...